We narrowed to 17,703 results for: SHE

1. 1996_pCCLsin.PPT.hPGK.EGFP-mCEP83.PRE

   Plasmid

   #231805

   Purpose

   Label the mother centrosome-specific distal appendage protein CEP83 by electroporating an mRNA encoding it as well as an eGFP fusion protein.

   Depositor

   Annette Oxenius

   Insert

   eGFP-mCEP83
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti puro HA-STX3

   Plasmid

   #227590

   Purpose

   Lentiviral plasmid expressing human STX3 protein

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   STX3 (STX3 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Promoter

   CMV

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-MM002-ZFP143-C-terminal-sgRNA

   Plasmid

   #212706

   Purpose

   Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and sgRNA targetting the C terminal of Zfp143 locus

   Depositor

   Elzo de Wit

   Insert

   ZFP143 C-terminal sgRNA (Zfp143 Mouse)
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR/hBIG1

   Plasmid

   #226251

   Purpose

   Entry plasmid of hBIG1 for Gateway system

   Depositor

   Hye-Won Shin

   Insert

   BIG1 (ARFGEF1 Human)
   

   Use

   Entry vector

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR/HA-hBIG2

   Plasmid

   #226252

   Purpose

   Entry plasmid of N-terminally HA-tagged hBIG2 for Gateway system

   Depositor

   Hye-Won Shin

   Insert

   BIG2 (ARFGEF2 Human)
   

   Use

   Entry vector

   Tags

   HA

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti puro HA-VIM

   Plasmid

   #227585

   Purpose

   Lentiviral plasmid expressing human VIM protein

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   VIM (VIM Human)
   

   Use

   Lentiviral

   Tags

   HA

   Promoter

   CMV

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598)

   Plasmid

   #225432

   Purpose

   Transcriptional unit of BeYDV Rep/RepA (C1:C2)

   Depositor

   Diego Orzaez

   Insert

   pNOS:Rep/RepA:tNOS
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN_TT DNM1L-V5

   Plasmid

   #220359

   Purpose

   Gateway entry vector for an inducible V5-tagged DNM1L

   Depositor

   Kevin Janes

   Insert

   DNM1L (DNM1L Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   V5

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.SLC17A2-flag

   Plasmid

   #224419

   Purpose

   Expresses SLC17A2 in mammalian cells

   Depositor

   Jonathan Long

   Insert

   Solute carrier family 17 (sodium phosphate), member 2 (SLC17A2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CD55-Delta3

    Plasmid

    #220872

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-3
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-3, short consensus repeat (SCR) domains 1, …

    Promoter

    CMV

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CD55-Delta4

    Plasmid

    #220873

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-4
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-4, short consensus repeat (SCR) domains 1, …

    Promoter

    CMV

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CD55-Delta1

    Plasmid

    #220870

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-1
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-1, the first short consensus repeat (SCR) d…

    Promoter

    CMV

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CD55-Delta2

    Plasmid

    #220871

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-2
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-2, short consensus repeat (SCR) domains 1 a…

    Promoter

    CMV

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. hs-cre::EBD, UAS-dBrainbow attB

    Plasmid

    #220326

    Purpose

    expresses a heat-shock inducible cre::EBD fusion protein and UAS-dBrainbow

    Depositor

    Erdem Bangi

    Inserts

    Cre::EBD (with intron)
    dBrainbow
    

    Expression

    Insect

    Promoter

    UAS and hs

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYES2/NTA/Leu-LegA7Δank

    Plasmid

    #216519

    Purpose

    Expression of LegA7 ΔAnk

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔAnk
    

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 331-454

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYES2/NTA/Leu-LegA7ΔAnk290

    Plasmid

    #216520

    Purpose

    Expression of LegA7 ΔAnk290

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔAnk290
    

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 290-454

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYES2/NTA/Leu-LegA7ΔNAnk264

    Plasmid

    #216521

    Purpose

    Expression of LegA7 ΔNAnk264

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔNAnk264
    

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 264-361

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYES2/NTA/Leu-LegA7ΔNAnk290

    Plasmid

    #216522

    Purpose

    Expression of LegA7 ΔNAnk290

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔNAnk290
    

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 290-361

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-RPL3L-TwStrep

    Plasmid

    #208649

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949

    Depositor

    Quanfu Ma

    Insert

    human RPL3L (ENST00000268661) (RPL3L Human)
    

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    contain rs753338989, rs746044959, rs752134949, pr…

    Promoter

    T7

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-CAG-K-red-SPOTIT(R271F)

    Plasmid

    #191494

    Purpose

    Red fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

    Depositor

    Wenjing Wang

    Insert

    K-red-SPOTIT(R271F)
    

    Use

    AAV

    Mutation

    R271F in OR

    Promoter

    CAG

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET50_G495R MDA5

    Plasmid

    #216803

    Purpose

    Expresses a single point mutant of melanoma differentiation-associated protein 5

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 (IFIH1 Human)
    

    Expression

    Bacterial

    Mutation

    G495R

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTRIPZ-NUDT17-C-HA-Puro

    Plasmid

    #195182

    Purpose

    Lentiviral vector for DOX-inducible NUDT17 gene expression with C-terminal HA tag. Gateway expression system. Selection by puromycin.

    Depositor

    Stephanie Roessler

    Insert

    NUDT17 (NUDT17 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV promoter and minimal CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pT7CFE1_polio_3ABC_C147R

    Plasmid

    #212985

    Purpose

    Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Poliovirus Type I Mahoney 3ABC C147R
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Mutation

    cysteine 147 in 3C changed to arginine

    Promoter

    T7

    Available Since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pT7CFE1_EVA71_VP12A_C110A

    Plasmid

    #212976

    Purpose

    Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Enterovirus A71 4643 VP12A C110A
    

    Use

    Mammalian cell free protein expression

    Tags

    HA tag

    Mutation

    changed cysteine 110 from 2A to alanine

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT7CFE1_polio_VP12A_C109R

    Plasmid

    #212987

    Purpose

    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Poliovirus Type I Mahoney VP12A C109R
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Mutation

    Cysteine 109 from 2A mutated to arginine

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCHA_SBV_NSs

    Plasmid

    #208938

    Purpose

    Expresses C-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells

    Depositor

    Gerald Barry

    Insert

    NSs
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted 47 bp at 5' end and 510 bp from 3&#x…

    Promoter

    CMV

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28-Flag-RBM24-95~236

    Plasmid

    #208634

    Purpose

    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236

    Depositor

    Quanfu Ma

    Insert

    human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    only aa 95~236, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAGW-Fray

    Plasmid

    #203979

    Purpose

    GFP-Fray under actin promoter for cell culture

    Depositor

    Andreas Jenny

    Insert

    Frayed (fray Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAMW-WnkAAxV

    Plasmid

    #203980

    Purpose

    Myc6 Wnk under actin promoter for cell culture

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Tags

    6xMyc

    Expression

    Insect

    Mutation

    RFxV mutated to AAxV

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. lenti-Cas9-Exo-Blast-BFP

    Plasmid

    #196721

    Purpose

    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-Exo1-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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