We narrowed to 22,272 results for: Sis
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Plasmid#188594PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K187A]
Plasmid#188593PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationK187A [AAA=> GCA]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pKLV2-EF1a-ArgSen (-)P2A (-)NLS
Plasmid#184690PurposeLentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLSDepositorInsertArgSen (-)P2A (-)NLS
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-ArgSen (-)NLS
Plasmid#184698PurposeLentiviral transfer plasmid for expression of ArgSen (-)NLSDepositorInsertArgSen (-)NLS
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-ArgSen (-)P2A (-)NLS
Plasmid#184699PurposeLentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLSDepositorInsertArgSen (-)P2A (-)NLS
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1373_mU6_sgRNA targeting e1
Plasmid#190684PurposesgRNA targeting enhancer 1 of MYCDepositorInsertsgRNA targeting enhancer 1 of MYC
UseCRISPR, Lentiviral, and Synthetic BiologyTagsBFP and PuromycinExpressionMammalianMutationPromotermouse U6 promoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5004_hU6_sgRNA targeting e7
Plasmid#190687PurposesgRNA targeting enhancer 7 of MYCDepositorInsertsgRNA targeting enhancer 7 of MYC
UseCRISPR, Lentiviral, and Synthetic BiologyTagsBFP and PuromycinExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5004_hU6_sgRNA targeting e4
Plasmid#190686PurposesgRNA targeting enhancer 4 of MYCDepositorInsertsgRNA targeting enhancer 4 of MYC
UseCRISPR, Lentiviral, and Synthetic BiologyTagsBFP and PuromycinExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1373_mU6_sgRNA targeting e3
Plasmid#190685PurposesgRNA targeting enhancer 3 of MYCDepositorInsertsgRNA targeting enhancer 3 of MYC
UseCRISPR, Lentiviral, and Synthetic BiologyTagsBFP and PuromycinExpressionMammalianMutationPromotermouse U6 promoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYI006
Plasmid#170840PurposeFRO6p::GUSPlus::tOCS in pFP100DepositorInsertFRO6p::GUSPlus::tOCS
UseTagsExpressionPlantMutationPromoterFRO6Available sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYI046
Plasmid#170842Purpose(pUBQ10::SMT2-FLAG::tOCS)DepositorInsertpUBQ10::SMT2-FLAG::tOCS
UseTagsExpressionPlantMutationPromoterpUBQ10Available sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIALD2S
Plasmid#185865PurposeKnocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiaeDepositorInsertALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_miR-290-295KO-gRNA3
Plasmid#172711PurposeCas9 with 5' gRNA for paired CRISPR KO of miR-290-295 clusterDepositorInsertmiR-290-295
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFAP4_BHLH_5-1
Plasmid#185054PurposeDeletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2DepositorInsertTFAP4_5_1_gRNA (Tcfap4 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFAP4_BHLH_3-2
Plasmid#185055PurposeDeletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_5-1 or TFAP4_BHLH_5-2DepositorInsertTFAP4_3_2_gRNA (Tcfap4 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFAP4_BHLH_5-2
Plasmid#185056PurposeDeletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2DepositorInsertTFAP4_5_2_gRNA (Tcfap4 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_W403A_6xH
Plasmid#185712PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
UseTags6xHis and MBPExpressionBacterialMutationChanged W403 to APromoterT7Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pNB203
Plasmid#169114PurposeTESEC expression vector for mEGFP-Alr fusion constructDepositorInsertmEGFP-alr (alr Mycobacterium tuberculosis H37Rv, Synthetic)
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E. coliPromoterpBADAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB204
Plasmid#169115PurposeTESEC expression vector for 6xHis-Alr fusion constrcutDepositorInsert6xHis-alr (alr Mycobacterium tuberculosis H37Rv, Synthetic)
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for E. coliPromoterpBADAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-β-actin3'UTR2
Plasmid#183717PurposetdTomato with fragment nucleotides 441-676 of beta-actin 3'UTR inserted in 3'UTRDepositorInserttdTomato with fragment (nucleotides 441-676) of mouse Actb 3'UTR
UseLentiviralTagsExpressionMutationPromoterUbiCAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdTomato--β-actin CDS2
Plasmid#183714PurposetdTomato with fragment nucleotides 398-818 of beta-actin coding sequence inserted in 3'UTRDepositorInserttdTomato with fragment (nucleotides 398-818) of mouse Actb coding dsequence
UseLentiviralTagsExpressionMutationPromoterUbiCAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-β-actin3'UTR1
Plasmid#183716PurposetdTomato with fragment nucleotides 1-440 of beta-actin 3'UTR inserted in 3'UTRDepositorInserttdTomato with fragment (nucleotides 1-440) of mouse Actb 3'UTR
UseLentiviralTagsExpressionMutationPromoterUbiCAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-β-actinCDS3
Plasmid#183715PurposetdTomato with fragment nucleotides 801-1127 of beta-actin coding sequence inserted in 3'UTRDepositorInserttdTomato with fragment (nucleotides 801-1127) of mouse Actb coding dsequence
UseLentiviralTagsExpressionMutationPromoterUbiCAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
MBP_Lims (MBP_LIM1234)
Plasmid#182042PurposeFor expression of human paxillin LIM domains 1-4 with N-terminal GST fusion protein in E. coli.DepositorInsertLIM1234 (PXN Human)
UseTagsMBPExpressionBacterialMutationLIM domains 1-4 onlyPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST_LIM234
Plasmid#182039PurposeFor expression of human paxillin LIM domains 2-4 with N-terminal GST fusion protein in E. coli.DepositorInsertLIM234 (PXN Human)
UseTagsGSTExpressionBacterialMutationLIM domains 2-4 onlyPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/MACROD1
Plasmid#172578PurposeFor generation of FlpIn lines with untagged MACROD1DepositorInsertMACROD1 (MACROD1 Human)
UseTagsnoneExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MCS-BirA(R118G)-HA-TARG1
Plasmid#172583PurposeFor transient expression of C-terminal BirA-tagged TARG1DepositorInsertTARG1 (OARD1 Human)
UseTagsBirA(R118G)-HAExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNanos3-del4-20_X
Plasmid#147945PurposeMammalian Expression of HsNanos3-del4-20DepositorInsertHsNanos3-del4-20 (NANOS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Ub-VPS13D
Plasmid#176462Purposeexpressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13DDepositorInsertVPS13D (VPS13D Human)
UseTagsGFP-UbExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Ub-VPS13D N3521S
Plasmid#176463Purposeexpressing VPS13D patient mutant N3521S in mammalian cellsDepositorInsertVPS13D (VPS13D Human)
UseTagsGFP-UbExpressionMammalianMutationN3521S mutationPromoterCMVAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-14
Plasmid#160714PurposeFor C-terminal tags with Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL) bordered by BsaI restriction sitesDepositorInsertTrichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B-mut1_E
Plasmid#146203PurposeInsect Expression of DmMe31B-mut1DepositorInsertDmMe31B-mut1 (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-xylA
Plasmid#158611PurposeLow phosphate inducible gRNA silencing the xylA promoterDepositorInsertxylA guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-udhA-xylA
Plasmid#158612PurposeLow phosphate inducible gRNA array to silence the xylA and udhA promotersDepositorInsertudhA-xylA guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA2-xylA
Plasmid#158613PurposeLow phosphate inducible gRNA array to silence the xylA and gltA2 promotersDepositorInsertgltA2-xylA guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-zwf-xylA
Plasmid#158614PurposeLow phosphate inducible gRNA array to silence the xylA and zwf promotersDepositorInsertzwf-xylA guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mSpata33-PA
Plasmid#179129PurposeExpression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertspermatogenesis associated 33 (Spata33 Mouse)
UseTagsPAExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-epELM-5
Plasmid#179391PurposeExpress a peptide (reside from 550 through 600) of human ELMSAN1 (Q6PJG2) in bacteriaDepositorInsertELMSAN1 (MIDEAS Human)
UseTags6x HisExpressionBacterialMutationPromoterT7 lacAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B K88E
Plasmid#175028PurposeExpresses WIPI2B (K88E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (K88E) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationLysine 88 to Glutamate (AAG to GAG)PromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B I92E
Plasmid#175029PurposeExpresses WIPI2B (I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (I92E) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationIsoleucine 92 to Glutamate (ATC to GAA)PromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B C93E
Plasmid#175030PurposeExpresses WIPI2B (C93E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (C93E) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationCysteine 93 to Glutamate (TGC to GAG)PromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B K128E
Plasmid#175031PurposeExpresses WIPI2B (K128E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (K128E) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationLysine 128 to Glutamate (AAG to GAG)PromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCm N-term MBP SMT3 NikJC199U
Plasmid#174363PurposeExpression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, MBP, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD2KN0899
Plasmid#177092PurposeMoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrSTR, CrSLS, CrLAMT, CrTDC, and Cr7-DLH driven by 4x opaatBDepositorInsertCrSTR, CrSLS, CrLAMT, CrTDC, and Cr7-DLH
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD2KN0895
Plasmid#177091PurposeMoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS driven by 4x opaatBDepositorInsertCrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQDPKN0892
Plasmid#177089PurposeMoClo Level P, position 1, plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, CrG8H, CrGOR, CrIO, and Cr7-DLGT driven by 4x opaatBDepositorInsertCrISY, NmMLPL, CrG8H, CrGOR, CrIO, and Cr7-DLGT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0865
Plasmid#177084PurposeMoClo Level 1, position 6, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31DepositorInsertgeraniol 8-oxidase (CrG8H) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0802
Plasmid#177083PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of strictosidine synthase (CrSTR) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31DepositorInsertstrictosidine synthase (CrSTR) from Catharanthus roseus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only