We narrowed to 22,272 results for: Sis

1. pAFMW-Nbr[R272A]

   Plasmid

   #188594

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   R272A [CGG=> GCG]

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAFMW-Nbr[K187A]

   Plasmid

   #188593

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   K187A [AAA=> GCA]

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAFMW-Nbr

   Plasmid

   #188584

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   deleted first ATG

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKLV2-EF1a-ArgSen (-)P2A (-)NLS

   Plasmid

   #184690

   Purpose

   Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   ArgSen (-)P2A (-)NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-EF1a-ArgSen (-)NLS

   Plasmid

   #184698

   Purpose

   Lentiviral transfer plasmid for expression of ArgSen (-)NLS

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   ArgSen (-)NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-EF1a-ArgSen (-)P2A (-)NLS

   Plasmid

   #184699

   Purpose

   Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   ArgSen (-)P2A (-)NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSLQ1373_mU6_sgRNA targeting e1

   Plasmid

   #190684

   Purpose

   sgRNA targeting enhancer 1 of MYC

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNA targeting enhancer 1 of MYC
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLQ5004_hU6_sgRNA targeting e7

   Plasmid

   #190687

   Purpose

   sgRNA targeting enhancer 7 of MYC

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNA targeting enhancer 7 of MYC
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSLQ5004_hU6_sgRNA targeting e4

   Plasmid

   #190686

   Purpose

   sgRNA targeting enhancer 4 of MYC

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNA targeting enhancer 4 of MYC
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLQ1373_mU6_sgRNA targeting e3

    Plasmid

    #190685

    Purpose

    sgRNA targeting enhancer 3 of MYC

    Depositor

    Stanley Qi

    Article

    Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

    Insert

    sgRNA targeting enhancer 3 of MYC
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    BFP and Puromycin

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYI006

    Plasmid

    #170840

    Purpose

    FRO6p::GUSPlus::tOCS in pFP100

    Depositor

    Christian Fankhauser

    Article

    Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

    Insert

    FRO6p::GUSPlus::tOCS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    FRO6

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYI046

    Plasmid

    #170842

    Purpose

    (pUBQ10::SMT2-FLAG::tOCS)

    Depositor

    Christian Fankhauser

    Article

    Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

    Insert

    pUBQ10::SMT2-FLAG::tOCS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIALD2S

    Plasmid

    #185865

    Purpose

    Knocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    ALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX458_miR-290-295KO-gRNA3

    Plasmid

    #172711

    Purpose

    Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    miR-290-295
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TFAP4_BHLH_5-1

    Plasmid

    #185054

    Purpose

    Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    TFAP4_5_1_gRNA (Tcfap4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFAP4_BHLH_3-2

    Plasmid

    #185055

    Purpose

    Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_5-1 or TFAP4_BHLH_5-2

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    TFAP4_3_2_gRNA (Tcfap4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TFAP4_BHLH_5-2

    Plasmid

    #185056

    Purpose

    Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    TFAP4_5_2_gRNA (Tcfap4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 2Bc-T MBP_BoMoC(ed)_W403A_6xH

    Plasmid

    #185712

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed W403 to A

    Promoter

    T7

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NK72

    Plasmid

    #176608

    Purpose

    To test macrophage engulfment initiation. Lacks the FcyR chain.

    Depositor

    Meghan Morrissey

    Article

    Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

    Insert

    CD86 (CD86 Human)
    

    Use

    Lentiviral

    Tags

    HA, SNAP-tag and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNB203

    Plasmid

    #169114

    Purpose

    TESEC expression vector for mEGFP-Alr fusion construct

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    mEGFP-alr (alr Mycobacterium tuberculosis H37Rv, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNB204

    Plasmid

    #169115

    Purpose

    TESEC expression vector for 6xHis-Alr fusion constrcut

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    6xHis-alr (alr Mycobacterium tuberculosis H37Rv, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. tdTomato-β-actin3'UTR2

    Plasmid

    #183717

    Purpose

    tdTomato with fragment nucleotides 441-676 of beta-actin 3'UTR inserted in 3'UTR

    Depositor

    Jeffrey Gerst

    Article

    Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

    Insert

    tdTomato with fragment (nucleotides 441-676) of mouse Actb 3'UTR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. tdTomato--β-actin CDS2

    Plasmid

    #183714

    Purpose

    tdTomato with fragment nucleotides 398-818 of beta-actin coding sequence inserted in 3'UTR

    Depositor

    Jeffrey Gerst

    Article

    Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

    Insert

    tdTomato with fragment (nucleotides 398-818) of mouse Actb coding dsequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. tdTomato-β-actin3'UTR1

    Plasmid

    #183716

    Purpose

    tdTomato with fragment nucleotides 1-440 of beta-actin 3'UTR inserted in 3'UTR

    Depositor

    Jeffrey Gerst

    Article

    Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

    Insert

    tdTomato with fragment (nucleotides 1-440) of mouse Actb 3'UTR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. tdTomato-β-actinCDS3

    Plasmid

    #183715

    Purpose

    tdTomato with fragment nucleotides 801-1127 of beta-actin coding sequence inserted in 3'UTR

    Depositor

    Jeffrey Gerst

    Article

    Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

    Insert

    tdTomato with fragment (nucleotides 801-1127) of mouse Actb coding dsequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MBP_Lims (MBP_LIM1234)

    Plasmid

    #182042

    Purpose

    For expression of human paxillin LIM domains 1-4 with N-terminal GST fusion protein in E. coli.

    Depositor

    Vesa Hytönen

    Article

    Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

    Insert

    LIM1234 (PXN Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    LIM domains 1-4 only

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GST_LIM234

    Plasmid

    #182039

    Purpose

    For expression of human paxillin LIM domains 2-4 with N-terminal GST fusion protein in E. coli.

    Depositor

    Vesa Hytönen

    Article

    Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

    Insert

    LIM234 (PXN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    LIM domains 2-4 only

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5/FRT/TO/MACROD1

    Plasmid

    #172578

    Purpose

    For generation of FlpIn lines with untagged MACROD1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-MCS-BirA(R118G)-HA-TARG1

    Plasmid

    #172583

    Purpose

    For transient expression of C-terminal BirA-tagged TARG1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    TARG1 (OARD1 Human)
    

    Use

    Tags

    BirA(R118G)-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-MS2-HA-HsNanos3-del4-20_X

    Plasmid

    #147945

    Purpose

    Mammalian Expression of HsNanos3-del4-20

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    HsNanos3-del4-20 (NANOS3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGFP-Ub-VPS13D

    Plasmid

    #176462

    Purpose

    expressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13D

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Use

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGFP-Ub-VPS13D N3521S

    Plasmid

    #176463

    Purpose

    expressing VPS13D patient mutant N3521S in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Use

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    N3521S mutation

    Promoter

    CMV

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTrichoGate-14

    Plasmid

    #160714

    Purpose

    For C-terminal tags with Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL) bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAc5.1B-lambdaN-HA-DmMe31B-mut1_E

    Plasmid

    #146203

    Purpose

    Insect Expression of DmMe31B-mut1

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmMe31B-mut1 (me31B Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCASCADE-xylA

    Plasmid

    #158611

    Purpose

    Low phosphate inducible gRNA silencing the xylA promoter

    Depositor

    Michael Lynch

    Article

    Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

    Insert

    xylA guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCASCADE-udhA-xylA

    Plasmid

    #158612

    Purpose

    Low phosphate inducible gRNA array to silence the xylA and udhA promoters

    Depositor

    Michael Lynch

    Article

    Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

    Insert

    udhA-xylA guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCASCADE-gltA2-xylA

    Plasmid

    #158613

    Purpose

    Low phosphate inducible gRNA array to silence the xylA and gltA2 promoters

    Depositor

    Michael Lynch

    Article

    Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

    Insert

    gltA2-xylA guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCASCADE-zwf-xylA

    Plasmid

    #158614

    Purpose

    Low phosphate inducible gRNA array to silence the xylA and zwf promoters

    Depositor

    Michael Lynch

    Article

    Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

    Insert

    zwf-xylA guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-mSpata33-PA

    Plasmid

    #179129

    Purpose

    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    spermatogenesis associated 33 (Spata33 Mouse)
    

    Use

    Tags

    PA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET-epELM-5

    Plasmid

    #179391

    Purpose

    Express a peptide (reside from 550 through 600) of human ELMSAN1 (Q6PJG2) in bacteria

    Depositor

    Shiro Iuchi

    Article

    Iuchi et al Commun Biol. 2021 Dec 15;4(1):1399. doi:

    Insert

    ELMSAN1 (MIDEAS Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Halo-WIPI2B K88E

    Plasmid

    #175028

    Purpose

    Expresses WIPI2B (K88E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

    Depositor

    Andrea Stavoe

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2B (K88E) (WIPI2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Lysine 88 to Glutamate (AAG to GAG)

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Halo-WIPI2B I92E

    Plasmid

    #175029

    Purpose

    Expresses WIPI2B (I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

    Depositor

    Andrea Stavoe

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2B (I92E) (WIPI2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Isoleucine 92 to Glutamate (ATC to GAA)

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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43. Halo-WIPI2B C93E

    Plasmid

    #175030

    Purpose

    Expresses WIPI2B (C93E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

    Depositor

    Andrea Stavoe

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2B (C93E) (WIPI2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Cysteine 93 to Glutamate (TGC to GAG)

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Halo-WIPI2B K128E

    Plasmid

    #175031

    Purpose

    Expresses WIPI2B (K128E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

    Depositor

    Andrea Stavoe

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2B (K128E) (WIPI2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Lysine 128 to Glutamate (AAG to GAG)

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCm N-term MBP SMT3 NikJC199U

    Plasmid

    #174363

    Purpose

    Expression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

    Depositor

    Brandon Greene

    Article

    Caceres et al Methods Enzymol. 2022;662:119-141. doi:

    Insert

    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    

    Use

    Tags

    8xHis, MBP, SMT3, and TEV

    Expression

    Bacterial

    Mutation

    C199U

    Promoter

    T7

    Available since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pEPQD2KN0899

    Plasmid

    #177092

    Purpose

    MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrSTR, CrSLS, CrLAMT, CrTDC, and Cr7-DLH driven by 4x opaatB

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    CrSTR, CrSLS, CrLAMT, CrTDC, and Cr7-DLH
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pEPQD2KN0895

    Plasmid

    #177091

    Purpose

    MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS driven by 4x opaatB

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pEPQDPKN0892

    Plasmid

    #177089

    Purpose

    MoClo Level P, position 1, plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, CrG8H, CrGOR, CrIO, and Cr7-DLGT driven by 4x opaatB

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    CrISY, NmMLPL, CrG8H, CrGOR, CrIO, and Cr7-DLGT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pEPQD1CB0865

    Plasmid

    #177084

    Purpose

    MoClo Level 1, position 6, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geraniol 8-oxidase (CrG8H) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pEPQD1CB0802

    Plasmid

    #177083

    Purpose

    MoClo Level 1, position 1, transcriptional unit for transient expression of strictosidine synthase (CrSTR) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    strictosidine synthase (CrSTR) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge