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We narrowed to 23,223 results for: Sis

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Showing: 11451 - 11500 of 23223 results

  6. mE-mCherry2A

    Plasmid
    #236248
    Purpose
    Middle entry fluorescent mCherry reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately
    Depositor
    James Amatruda, Genevieve Kendall
    Article
    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10
    Insert
    mCherry2A
    Expression
    Mammalian
    Mutation
    Added a viral2A sequence to mCherry with no stop …
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236248-plasmid-map-sequence-id-486041

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  7. mE-GFP2A

    Plasmid
    #236247
    Purpose
    Middle entry fluorescent GFP reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately
    Depositor
    James Amatruda, Genevieve Kendall
    Article
    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10
    Insert
    eGFP
    Expression
    Mammalian
    Mutation
    Added a viral2A sequence with no stop codon
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236247-plasmid-map-sequence-id-486040

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  8. pEmal10H – MBP-EWS_300-447 (RGG1+RRM)

    Plasmid
    #238369
    Purpose
    Expresses the RGG1-RRM (300-447) region from the RNA-binding protein EWS
    Depositor
    David Libich
    Insert
    EWSR1 (EWSR1 Human)
    Tags
    10xHis-MBP
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-299, 448-638
    Promoter
    T7
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    238369-plasmid-map-sequence-id-478743

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  9. pEmal10H – MBP-EWS_549-638 (RGG3)

    Plasmid
    #238370
    Purpose
    Expresses the RGG3 (549-638) region from the RNA-binding protein EWS
    Depositor
    David Libich
    Insert
    EWSR1 (EWSR1 Human)
    Tags
    10xHis-MBP
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-548
    Promoter
    T7
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    238370-plasmid-map-sequence-id-478744

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  10. pUC19_21 tRNA genes

    Plasmid
    #242430
    Purpose
    In vitro transcription of 21 tRNAs after Nt.BspQI treatment. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene.
    Depositor
    Sebastian Maerkl
    Article
    Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:
    Insert
    21 tRNA genes with a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene
    Expression
    Bacterial
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    242430-plasmid-map-sequence-id-485004

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  13. pmEos3.1-N1 GBP

    Plasmid
    #220492
    Purpose
    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos3.1 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy
    Depositor
    Frederic Meunier
    Article
    Gormal et al Nat Protoc. 2025 Aug 8. doi: 10.1038/s41
    Insert
    GFP Binding Protein tagged with mEos3.1
    Tags
    mEos3.1
    Expression
    Mammalian
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    220492-plasmid-map-sequence-id-442019

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  14. pGTPA-Gm

    Plasmid
    #171527
    Purpose
    Plasmid contains the Cas12k gRNA and transposon donor cassette assembled with gentamycin-resistant gene
    Depositor
    Quanjiang Ji
    Article
    Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:
    Insert
    Cas12k gRNA, transposon donor cassette, gentamycin-resistant gene
    Use
    CRISPR
    Promoter
    Constitutive promoter J23119
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    171527-plasmid-map-sequence-id-340981

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  16. pEPHS1CB0027

    Plasmid
    #227570
    Purpose
    Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS
    Depositor
    Nicola Patron
    Article
    Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:
    Insert
    35Sshort_TMV_TkTXSS (T374G/E379D/Q486H/A745P)_35S
    Expression
    Plant
    Mutation
    T374G / E379D / Q486H / A745P, BsaI sites removed…
    Promoter
    35Sshort_TMV (pICH51277)
    Available Since
    July 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    227570-plasmid-map-sequence-id-455283

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  17. pHS1625

    Plasmid
    #239834
    Purpose
    OrufIscB-REC E. coli expression for recombinant purification
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    OrufIscB-REC
    Tags
    14xHis-TwinStrep-bdSUMO
    Expression
    Bacterial
    Mutation
    Insertion of REC domain from NbaCas9-1
    Promoter
    T7
    Available Since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    239834-plasmid-map-sequence-id-479361

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  22. pHS2096

    Plasmid
    #239835
    Purpose
    NovaIscB E. coli expression for recombinant purification
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    NovaIscB
    Tags
    14xHis-TwinStrep-bdSUMO
    Expression
    Bacterial
    Mutation
    Insertion of REC domain from NbaCas9-1 and swap o…
    Promoter
    T7
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239835-plasmid-map-sequence-id-479109

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  24. pBN201

    Plasmid
    #217917
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutations
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2025 Apr 11;16(1):3437. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutations
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, Y715E, Y717E, Y718E
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    217917-plasmid-map-sequence-id-434787

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  25. pBN363

    Plasmid
    #217918
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E and N711Y mutations
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2025 Apr 11;16(1):3437. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E and N711Y mutations
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, Y715E, Y717E, Y718E, N711Y
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    217918-plasmid-map-sequence-id-434785

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  26. pBN413

    Plasmid
    #217919
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E and N711Y mutations
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2025 Apr 11;16(1):3437. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E and N711Y mutations
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y715E, Y717E, Y718E, N711Y
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    217919-plasmid-map-sequence-id-434784

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  48. pYihI-N-term D-N/E-Q

    Plasmid
    #233095
    Purpose
    Expression of GST-YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
    Depositor
    Michael Downey
    Article
    Baijal et al bioRxiv 2025.02.12.637445
    Insert
    YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    N-term aspartic acids (D) mutated to asparagine (…
    Available Since
    May 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233095-plasmid-map-sequence-id-468389

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  49. pYihI-N-term D-A/E-L

    Plasmid
    #233097
    Purpose
    Expression of GST-YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
    Depositor
    Michael Downey
    Article
    Baijal et al bioRxiv 2025.02.12.637445
    Insert
    YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    N-term aspartic acids (D) mutated to alanine (A) …
    Available Since
    May 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233097-plasmid-map-sequence-id-468390

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Showing: 11451 - 11500 of 23223 results