We narrowed to 7,293 results for: 11

1. (attB) tdMCP-tagged BRaf

   Plasmid

   #226165

   Purpose

   Used to integrate 3xFlag-tdMCP-BRaf without MS2-circRNA barcdes into landing pad cell lines containing a single promoter.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Insert

   3xFlag-tdMCP-BRaf (BRAF Human)
   

   Use

   Bxb1 attb, no promoter

   Tags

   3xFlag-tdMCP and IRES-mCherry-P2A-PuroR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCKC017

   Plasmid

   #226622

   Purpose

   pLenti wt hTdT template for libraries 1-2

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Insert

   Terminal deoxynucleotidyl transferase (DNTT Human)
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Mutation

   synonymous mutation in D2, synonymous mutation in…

   Promoter

   CMV

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-MCHpr-GCaMP6f

   Plasmid

   #221039

   Purpose

   Expresses GCaMP6f under the rat pro-melanin-concentrating hormone promoter

   Depositor

   Yanhua Huang

   Article

   Guo et al Biol Psychiatry. 2022 Dec 1;92(11):880-8

   Insert

   GCaMP6f
   

   Use

   AAV; Rat targeting

   Expression

   Mammalian

   Mutation

   A317E

   Promoter

   Rat MCH

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTrcHis_VectorA_short_speGpromoter_SAT1 (human)

   Plasmid

   #220477

   Purpose

   heterologous expression of SSAT1 under endogenous speG E. coli promoter

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   SSAT1 (SAT1 Human)
   

   Expression

   Bacterial

   Mutation

   none

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNeonGreen-C1-Nir1-631-894

   Plasmid

   #220089

   Purpose

   Truncated Nir1-LNS2 domain lacking N-terminal amphipathic helix; loses ability to bind PA

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Tags

   NeonGreen-GGSGG

   Expression

   Mammalian

   Mutation

   amino acids 631-894

   Promoter

   CMV

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNeonGreen-N1-Nir1-613-630

   Plasmid

   #220088

   Purpose

   N-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PA

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Tags

   GGSGG-NeonGreen

   Expression

   Mammalian

   Mutation

   amino acids 613-630

   Promoter

   CMV

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-syn-F14F15S-jGCaMP7s

   Plasmid

   #178514

   Purpose

   AAV vector for Flpo recombinase dependent jGCaMP7s

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   jGCaMP7s
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-syn-mCre

   Plasmid

   #178515

   Purpose

   AAV vector for mCre recombinase

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   mCre
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. WT_IFL_G1A-T2A_R

   Plasmid

   #200183

   Purpose

   WT PA14 I-F Leader and G1A-T2A Repeat

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A)
   

   Expression

   Bacterial

   Mutation

   G1A-T2A in the I-F repeat

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. WT_IFL_G1A-T2A-A28C_R

    Plasmid

    #200185

    Purpose

    WT PA14 I-F Leader and G1A-T2A-A28C Repeat

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
    

    Expression

    Bacterial

    Mutation

    G1A-T2A-A28C in the I-F repeat

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRAW-457

    Plasmid

    #200213

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas1 H25A mutation

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRAW-458

    Plasmid

    #200214

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas1 E184A mutation

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRAW-459

    Plasmid

    #200215

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas1 K214A mutation

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRAW-460

    Plasmid

    #200216

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 R55E-N56D mutations

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRAW-461

    Plasmid

    #200217

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 K11D-R12E mutations

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRAW-462

    Plasmid

    #200218

    Purpose

    Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    PaCas1, PaCas2-3
    

    Tags

    Strep II-TEV on Cas1

    Expression

    Bacterial

    Mutation

    Cas2 K11D-R12E, R55E-N56D mutations

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-myc-EphB4

    Plasmid

    #200983

    Purpose

    Mammalian expression plasmid for myc-tagged EphB4

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EphB4 (EPHB4 Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-SMS1-TEV-HAT

    Plasmid

    #202557

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    SGMS1 (SGMS1 Human)
    

    Tags

    Histidine-Affinity-tag (HAT) and TEV cleavable si…

    Expression

    Mammalian

    Mutation

    deleted stop codon (TAA)

    Promoter

    CAG and chicken β-actin promoter

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-MYO10-FERM-ITGBD

    Plasmid

    #194855

    Purpose

    Express EGFP-MYO10 with mutations (S2001_F2002insA/T2009D) that interfere with ITGB binding in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

    Insert

    MYO10 S2001_F2002insA/T2009D (MYO10 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    MYO10 has the following mutations S2001_F2002insA…

    Promoter

    CMV

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5-Myc-PSK2 (K57A)

    Plasmid

    #197118

    Purpose

    Expression of kinase-defective MYC-tagged PSK2 (K57A) / TAOK1 (K57A) in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

    Insert

    TAOK1 (K57A) (TAOK1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Changed K 57 to A

    Promoter

    SV40

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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