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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1

Plasmid

#188975

Purpose

A human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g1 guide RNA

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

Plasmid

#188976

Purpose

A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g2 guide RNA

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-pFOGc::RfA

Plasmid

#186397

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of the pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::RfA

Plasmid

#186396

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pENT-L3-a-element-L4

Plasmid

#186366

Purpose

Entry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequences

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

a-element early Otx neural enhancer

Use

Protein expression

Promoter

T7 promoter

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-Venus-stop-L2

Plasmid

#186360

Purpose

Entry clone with ORF encoding Venus fluorescent protein flanked by Gateway recombination sequences

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Venus yellow fluorescent protein

Use

Expression of the venus fluorescent protein

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGP8GF-ZmAFB2/3 b2

Plasmid

#188425

Purpose

Zea Mays Nuclear Auxin Signaling Receptor AFB2/3 b2

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor AFB2/3 b2

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGP8GF-ZmAFB2/3 a

Plasmid

#188423

Purpose

Zea Mays Nuclear Auxin Signaling Receptor AFB2/3 A

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor AFB2/3 a

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGP4G-REL2N91-ccdB

Plasmid

#188444

Purpose

Zea Mays Nuclear Auxin Signaling CoRepressor REL2N91 and pDEST

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

REL2N91/ccdB

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-ZmIAA8ΔPB1

Plasmid

#188432

Purpose

Zea Mays Nuclear Auxin Signaling Repressor IAA8

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Repressor IAA8deltaPB1

Use

Cloning

Mutation

deleted PB1 domain

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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PDEST-pSP172BSSPE-pFOGc::RfA-HA

Plasmid

#186406

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1-MyTH-FERM

Plasmid

#187370

Purpose

Expresses human Plekhh1-MyTH-FERM labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1-MyTH-FERM

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_MTOR

Plasmid

#183311

Purpose

All-in-One CRISPRko system with a guide RNA that targets MTOR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

MTOR

Use

Lentiviral

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_BRAF

Plasmid

#183273

Purpose

All-in-One CRISPRko system with a guide RNA that targets BRAF gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BRAF

Use

Lentiviral

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_CYP19A1

Plasmid

#183280

Purpose

All-in-One CRISPRko system with a guide RNA that targets CYP19A1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CYP19A1

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DHFR

Plasmid

#183281

Purpose

All-in-One CRISPRko system with a guide RNA that targets DHFR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DHFR

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FLT1

Plasmid

#183292

Purpose

All-in-One CRISPRko system with a guide RNA that targets FLT1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FLT1

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM262 - pDESTR4-R3 mosTI hygroR target

Plasmid

#159840

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI hygroR target

Expression

Worm

Mutation

Not applicable

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_YES1

Plasmid

#183329

Purpose

All-in-One CRISPRko system with a guide RNA that targets YES1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

YES1

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ROS1

Plasmid

#183320

Purpose

All-in-One CRISPRko system with a guide RNA that targets ROS1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ROS1

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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