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pDONR223_ERBB2IP_p.I979M

Plasmid

#81568

Purpose

Gateway Donor vector containing ERBB2IP , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2IP (ERBIN Human)

Use

Gateway Cloning

Mutation

T271P; D547G; I979M; E1205G; Q1226P; K1287R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-active WRN

Plasmid

#125788

Purpose

constitutive, ectopic expression of human active WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-active WRN (WRN Human)

Tags

5x myc

Expression

Mammalian

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN K577M

Plasmid

#125790

Purpose

constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN K577M (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

K577M (helicase-dead)

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN E84A

Plasmid

#125789

Purpose

constitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN E84A (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

E84A (exonuclease-dead)

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN E84A K577M

Plasmid

#125791

Purpose

constitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN E84A/K577M (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

E84A/K577M (exonuclease/helicase-dead)

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pFGC-I2Cas9

Plasmid

#173158

Purpose

Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.

Depositor

Piotr Ziolkowski

Article

Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

Inserts

hSpCas9
pNAP:dsRED:tNOS
pMAS:BAR:tMAS
ICU2 upstream regulatory region

Tags

3xFLAG-NLS and NLS

Expression

Plant

Mutation

Contains a D169N mutation with no visible effect …

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgMLH1-1

Plasmid

#125776

Purpose

constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgMLH1-1 (MLH1 Human)

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgMLH1-2

Plasmid

#125777

Purpose

constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgMLH1-2 (MLH1 Human)

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgWRN-EIJ

Plasmid

#125774

Purpose

constitutive expression of a guide RNA targeting an exon-intron junction of human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgWRN-EIJ (WRN Human)

Use

CRISPR

Available Since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shPSMD2

Plasmid

#125779

Purpose

constitutive expression of a short-hairpin RNA targeting human PSMD2 (RNAi positive control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shPSMD2 (PSMD2 Human)

Use

RNAi

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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