We narrowed to 9,400 results for: Coli

1. pJExpress401-KDAC8

   Plasmid

   #224208

   Purpose

   Expresses human KDAC8 (HDAC8) in E. coli

   Depositor

   Terry Watt

   Article

   Toro et al Protein Sci. 2015 Dec;24(12):2020-32. do

   Insert

   KDAC8 (HDAC8 Human)
   

   Use

   Tags

   TEV-cleavable His6

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available Since

   Dec. 6, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pLei-sfGFP-D134*

   Plasmid

   #191110

   Purpose

   An E. coli sfGFP reporter with one TAG at position 134

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   superfolder green fluorescence protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changing D134 to TAG

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLei-sfGFP-V2*D134*

   Plasmid

   #191111

   Purpose

   An E. coli sfGFP reporter with two TAG at positions 2 & 134

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   superfolder green fluorescence protein
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changing V2 & D134 to TAG

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-CsHPAD-AE

   Plasmid

   #226982

   Purpose

   Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase activating enzyme (HPAD-AE) in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   CsHPAD-AE
   

   Use

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-CsHPAD_beta-gamma

   Plasmid

   #226981

   Purpose

   Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   CsHPAD_beta-gamma
   

   Use

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-OuIAD(W392A)

   Plasmid

   #226980

   Purpose

   Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392A variant in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   OuIAD(W392A)
   

   Use

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Mutation

   W392A

   Promoter

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-OuIAD(W392F)

   Plasmid

   #226979

   Purpose

   Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392F variant in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   OuIAD(W392F)
   

   Use

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Mutation

   W392F

   Promoter

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-OuIAD(F401A)

   Plasmid

   #226978

   Purpose

   Expressing Olsenella uli indoleacetate decarboxylase (IAD) F401A variant in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   OuIAD(F401A)
   

   Use

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Mutation

   F401A

   Promoter

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-OuIAD(L616E)

   Plasmid

   #226977

   Purpose

   Expressing Olsenella uli indoleacetate decarboxylase (IAD) L616E variant in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   OuIAD(L616E)
   

   Use

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Mutation

   L616E

   Promoter

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-OuIAD(R226K)

    Plasmid

    #226976

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226K variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226K)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226K

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-OuIAD(R226M)

    Plasmid

    #226975

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226M variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226M)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226M

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-OuIAD(R226E)

    Plasmid

    #226974

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226E variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226E)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226E

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-OuIAD(H514E)

    Plasmid

    #226972

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514E variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(H514E)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    H514E

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-OuIAD(H514A)

    Plasmid

    #226971

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(H514A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    H514A

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-OuIAD(C500S)

    Plasmid

    #226970

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) C500S variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(C500S)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    C500S

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-OuIAD(G853A)

    Plasmid

    #226969

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) G853A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(G853A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    G853A

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-OuIAD-AE

    Plasmid

    #226968

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase activating enzyme (IAD-AE) in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD-AE
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET24a_SMOX

    Plasmid

    #186345

    Purpose

    C-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cells

    Depositor

    Sandra Gabelli

    Article

    Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

    Insert

    Spermine oxidase (SMOX Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJB65

    Plasmid

    #226319

    Purpose

    E. coli protein expression for protein purification

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    6xHis- (VTG to AAA MR1-6)-strep
    

    Use

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Mutation

    N-terminal domain deleted, C-terminal domain dele…

    Promoter

    T7

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJB55

    Plasmid

    #226310

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with V(T/S)G to AAA CsoS2 MR1-5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

    Promoter

    pTRC

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-VHH-ddFLN4-HIS-ybbR

    Plasmid

    #169137

    Purpose

    E. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    VHH
    

    Use

    Tags

    6x His tag, ddFLN4 tag, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a- mCherry-FIVAR-HIS-ybbR

    Plasmid

    #169138

    Purpose

    E. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    mCherry
    

    Use

    Tags

    6x Histag, FIVAR tag, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRSET-Cm-CspB

    Plasmid

    #215400

    Purpose

    Cm NCPPB382 cspB codon-optimized for E. coli recombinant protein expression (6xHis-CspB)

    Depositor

    Gitta Coaker

    Article

    Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

    Insert

    cspB
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSET-Cm-CspA2

    Plasmid

    #215399

    Purpose

    Cm NCPPB382 cspA2 codon-optimized for E. coli recombinant protein expression (6xHis-CspA2)

    Depositor

    Gitta Coaker

    Article

    Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

    Insert

    cspA2
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYPQ121-Split-TALED

    Plasmid

    #218171

    Purpose

    Express plastid TALE-linked deaminases (TALED), composed of TALE, split DddA originated from Burkholderia cenocepacia, and an engineered deoxyadenosine deaminase derived from the E. coli TadA protein

    Depositor

    Yiping Qi

    Article

    Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.

    Insert

    TALE-Intron-N-DddAtox half (G1397N)-T2A-TALE-C-DddAtox half (G1397C)-TadA8e
    

    Use

    Plastid base editing

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSD113A

    Plasmid

    #218133

    Purpose

    For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved degron SD56

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD56
    

    Use

    Tags

    rpoZ

    Expression

    Mutation

    Promoter

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BcC _pET29b

    Plasmid

    #215403

    Purpose

    Expression construct for BcC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    BcC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. OoH_pET29b

    Plasmid

    #215424

    Purpose

    Expression construct for OoH gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    OoH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Ph-NylC_pET28a

    Plasmid

    #215425

    Purpose

    Expression construct for Ph-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Ph-NylC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ReU_pET29b

    Plasmid

    #215426

    Purpose

    Expression construct for ReU gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    ReU
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge