We narrowed to 2,316 results for: pET28

1. pSumo24P7vB4

   Plasmid

   #113073

   Purpose

   Plasmid for highly efficient expression of engineered IL24 with binding affinity to cognate receptors

   Depositor

   Bohdan Schneider

   Article

   Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

   Insert

   Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 SUMO part (Brachypodium distachyon), Human)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

   Promoter

   T7

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSumo24P7

   Plasmid

   #113072

   Purpose

   Plasmid for highly efficient expression of engineered IL24 with mutated binding sites

   Depositor

   Bohdan Schneider

   Article

   Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

   Insert

   Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 SUMO part (Brachypodium distachyon), Human)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

   Promoter

   T7

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Δ1-60MEK1C222

   Plasmid

   #164641

   Purpose

   Bacterial expression plasmid for His6-ΔMEK1C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   S222C/C277S/C376S; residues 1-60 at N-terminus we…

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Δ1-60MEK1C218

   Plasmid

   #164643

   Purpose

   Bacterial expression plasmid for His6-ΔMEK1C218

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   S218C/C277S/C376S; residues 1-60 at N-terminus we…

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Δ1-60MEK1C218C222

   Plasmid

   #164645

   Purpose

   Bacterial expression plasmid for His6-ΔMEK1C218C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   S218C/S222C/C277S/C376S; residues 1-60 at N-termi…

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNICBio2_WDR5:2-334

   Plasmid

   #211027

   Purpose

   bacterial expression of human WDR5 fragment A2 to C334. N-terminal 10X His tag with TEV cleavage and C-terminal biotin acceptor peptide tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   WDR5:A2-C334 (WDR5 Human)
   

   Use

   Tags

   MHHHHHHHHHHDLGTENLYFQS and SSKGGYGLNDIFEAQKIEWHE

   Expression

   Bacterial

   Mutation

   wild type

   Promoter

   T7

   Available since

   April 4, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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