We narrowed to 10,182 results for: yeast
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Plasmid#185072PurposeBacterial expression of N-terminus and FXFG repeats of NUP1 nucleoporin as GST-Nup1-N+Rep fusionDepositorInsertNUP1
TagsGSTExpressionYeastMutationNup1 N-term+FXFG repeatsAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
HCP2
Plasmid#166104PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombinationDepositorAvailable SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP3
Plasmid#166105PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombinationDepositorAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP7
Plasmid#166109PurposeThis plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.DepositorAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
brr2-C520R pRS313
Plasmid#111412Purposebrr2-C520R (endogenous promoter)DepositorInsertBRR2
ExpressionYeastMutationC520R aa mutationAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
brr2-N1104A pRS313
Plasmid#111414Purposebrr2-N1104A (endogenous promoter)DepositorInsertBRR2
ExpressionYeastMutationN1104A aa mutationAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
brr2-R1107L pRS313
Plasmid#111416Purposebrr2-R1107L (endogenous promoter)DepositorInsertBRR2
ExpressionYeastMutationR1107L aa mutationAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pREP81xV5_ccdB
Plasmid#79019PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selctionDepositorTypeEmpty backboneTagsdouble V5 epitope tagExpressionYeastAvailable SinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP41xV5_ccdB
Plasmid#79017PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneTagsdouble V5 epitope tagExpressionYeastAvailable SinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP82xV5_ccdB
Plasmid#79020PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selectionDepositorTypeEmpty backboneTagsdouble V5 epitope tagExpressionYeastAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP42xV5_ccdB
Plasmid#79018PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneTagsdouble V5 epitope tagExpressionYeastAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK275
Plasmid#73363PurposeEncodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)DepositorInsertdelta-8 sphingolipid desaturase
ExpressionYeastMutationmissing residues 1-6PromoterGAL1Available SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
brr2-Q904E pRS313
Plasmid#111413Purposebrr2-Q904E (endogenous promoter)DepositorInsertBRR2
ExpressionYeastMutationQ904E aa mutationAvailabilityAcademic Institutions and Nonprofits only -
brr2-R1107A pRS313
Plasmid#111415Purposebrr2-R1107A (endogenous promoter)DepositorInsertBRR2
ExpressionYeastMutationR1107A aa mutationAvailabilityAcademic Institutions and Nonprofits only -
pGR595
Plasmid#213785PurposeIntegrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.DepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyExpressionYeastAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-RNQ1
Plasmid#181706PurposeGalactose inducible expression of RNQ1DepositorInsertRNQ1 (RNQ1 Budding Yeast)
ExpressionYeastAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mNeonGreen (ErmR)
Plasmid#177208PurposePlasmid with p15A origin for constitutive mNeonGreen expression in E. coliDepositorInsertmNeonGreen
ExpressionBacterialMutationYeast codon optimizedPromoterSynthetic constitutive promoterAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mNeonGreen (KmR)
Plasmid#177209PurposePlasmid with p15A origin for constitutive mNeonGreen expression in E. coliDepositorInsertmNeonGreen
ExpressionBacterialMutationYeast codon optimizedPromoterSynthetic constitutive promoterAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Kar2-Beta-Amyloid
Plasmid#181707PurposeGalactose inducible expression of Kar2-Beta-AmyloidDepositorInsertKar2-Beta-Amyloid
ExpressionYeastAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp204-PADH1-AFB2
Plasmid#99531PurposeS. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)DepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS303-PADH1-AFB2
Plasmid#99530PurposeS. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)DepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHflu4
Plasmid#203882PurposepHI1 with insertion of monomeric red fluorescent protein (mRFP)DepositorInsertmonomeric red fluorescent protein
UseSynthetic BiologyExpressionBacterial and YeastAvailable SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBHM2641
Plasmid#229525PurposeCarries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseCRISPRTagst2a-mNeonGreen-AID*ExpressionBacterial and YeastMutationF74GPromoterTEF1Available SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2647
Plasmid#229989PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2648
Plasmid#229990PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2649
Plasmid#229991PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
LHP2691 - pET30-Ub-K63A
Plasmid#32179DepositorTagsHISExpressionBacterialMutationK63APromoterT7Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
LHP2690 - pET30-Ub-K48A
Plasmid#32178DepositorTagsHISExpressionBacterialMutationK48APromoterT7Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
STK25
Plasmid#39075PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-TAF15
Plasmid#181713PurposeGalactose inducible expression of TAF15DepositorInsertTAF15 (TAF15 Human)
ExpressionYeastAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only