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We narrowed to 63,680 results for: cloning

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Showing: 4621 - 4640 of 63680 results

  1. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid
    #106965
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106965-plasmid-map-sequence-id-207982

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  3. pDHt/sk-PE

    Plasmid
    #92126
    Purpose
    Expression of codon-optimized Cas9-eGFP fusion protein with Ppdc promoter in Trichoderma reesei
    Depositor
    Zhihua Zhou
    Publication
    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-eGFP
    Use
    CRISPR; Trichoderma reesei expression
    Tags
    EGFP
    Promoter
    Ppdc
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92126-plasmid-map-sequence-id-195058

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  4. pBADTrfp

    Plasmid
    #99382
    Purpose
    This promoter system provided the highest RFP expression upon induction
    Depositor
    Nathan Hillson
    Insert
    RFP
    Expression
    Bacterial
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99382-plasmid-map-sequence-id-192484

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  8. pTetON-DeAct-SpvB

    Plasmid
    #89455
    Purpose
    Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells
    Depositor
    Brad Zuchero
    Publication
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DeAct-SpvB (spvB Salmonella enterica)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Truncation spanning SpvB amino acids 375-591 (mon…
    Promoter
    TRE3G
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89455-plasmid-map-sequence-id-176825

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  12. pHD-DsRed-attP-w+

    Plasmid
    #80898
    Purpose
    Vector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Inserts
    attP
    LoxP-3xP3-DsRed-LoxP
    GMR-white
    Use
    High copy, amp resistance
    Promoter
    3xP3 and GMR::hsp70
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80898-plasmid-map-sequence-id-172486

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  13. pCFJ1415

    Plasmid
    #84828
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 introns
    Depositor
    Andrew Fire
    Publication
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Expression
    Bacterial and Worm
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84828-plasmid-map-sequence-id-163670

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  14. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

    Plasmid
    #84245
    Purpose
    Expresses direct fusion KRAB-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    KRAB-tagBFP-Sp dCas9
    Puro
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), HA tag, KRAB, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84245-plasmid-map-sequence-id-160888

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  18. Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

    Plasmid
    #73164
    Purpose
    multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells
    Depositor
    Bruce Edgar
    Publication
    Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:
    Insert
    mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)
    Tags
    mRFP1, GFP
    Expression
    Insect
    Mutation
    Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73164-plasmid-map-sequence-id-134544

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Showing: 4621 - 4640 of 63680 results