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We narrowed to 6,501 results for: nls

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Showing: 4641 - 4660 of 6501 results

  3. pCS6-ZF(VEGFA)-StaPL(AI)-YFP-VPR

    Plasmid
    #111502
    Purpose
    Expresses a zinc finger specific to the human VEGFA locus, which is linked to a VPR transcriptional activation domain via a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].
    Depositor
    Michael Lin
    Publication
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    ZF(VEGFA)-StaPL(AI)-YFP-VPR
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries V36M, T54A, and S122…
    Promoter
    CMV
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111502-plasmid-map-sequence-id-214269

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  5. pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA

    Plasmid
    #113685
    Purpose
    An EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.
    Depositor
    Jonathan Ploski
    Publication
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    inverted de-catalyzed SaCas9
    Use
    AAV, CRISPR, Cre/Lox, and Synthetic Biology
    Tags
    NLS and VP64
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    113685-plasmid-map-sequence-id-232027

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  8. pSFFV-dNme2Cas9

    Plasmid
    #193190
    Purpose
    Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)
    Depositor
    Erik Sontheimer
    Publication
    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dNme2Cas9
    Tags
    NLS and NLS-3xHA-NLS
    Expression
    Mammalian
    Mutation
    H588A, D16A
    Promoter
    SFFV
    Available since
    Jan. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    193190-plasmid-map-sequence-id-381070

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  10. pLV-REPORT (PGK) NanoLuc

    Plasmid
    #208576
    Purpose
    Empty NanoLuc Lentiviral reporter
    Depositor
    David Bersten
    Publication
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    NanoLuciferase
    d2eGFP
    Empty response element
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    3x SV40 NLS and PEST
    Expression
    Mammalian
    Promoter
    PGK and miniP
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    208576-plasmid-map-sequence-id-413002

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  11. A3G-BE5.13

    Plasmid
    #163637
    Purpose
    C to T DNA base editing
    Depositor
    Xue Gao
    Publication
    Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    A3G-BE5.13
    Tags
    BPSV40 NLS
    Expression
    Mammalian
    Mutation
    See manuscript
    Promoter
    CMV
    Available since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    163637-plasmid-map-sequence-id-320316

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  13. pEMS1382

    Plasmid
    #29182
    Purpose
    Expression of the eGFP reporter gene was detected (positive) in the brain, but not detected (negative) in the eye.
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple183 (S100B Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Nov. 15, 2011
    Availability
    Academic Institutions and Nonprofits only
    29182-plasmid-map-sequence-id-13597

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  14. pPB9v1

    Plasmid
    #210028
    Purpose
    Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Publication
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210028-plasmid-map-sequence-id-413997

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  15. PX458-3xHA-en31FnCas9ABEmax8.17d

    Plasmid
    #201956
    Purpose
    Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Publication
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201956-plasmid-map-sequence-id-405311

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  16. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid
    #196722
    Purpose
    Tet-inducible expression of Cas9-T2A-miRFP670nano
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Promoter
    TRE, PGK
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196722-plasmid-map-sequence-id-389804

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  17. pmiCas9-msa

    Plasmid
    #164833
    Purpose
    miCas9-msa fusion protein expressing plasmid
    Depositor
    Eugene Chen
    Publication
    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miCas9-msa
    Use
    CRISPR
    Tags
    FLAG and NLS
    Expression
    Mammalian
    Mutation
    contains mSA sequence range is 5106-5447 and Brex…
    Promoter
    CMV
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164833-plasmid-map-sequence-id-329431

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  19. pKS7107

    Plasmid
    #89051
    Purpose
    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9
    Depositor
    Ervin Welker
    Publication
    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Nm crRNA
    Nm tracrRNA
    hNmCas9
    Use
    CRISPR
    Tags
    HA tag, NLS, and SV40 NLS
    Promoter
    EF1a and human U6
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    89051-plasmid-map-sequence-id-176334

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  20. AALN-BE4max

    Plasmid
    #138161
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(AALN)-nSpCas9-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 R33A + K34A + H122L + D124N; with…
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    138161-plasmid-map-sequence-id-272350

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Showing: 4641 - 4660 of 6501 results