We narrowed to 5,560 results for: Chia

1. pHCKan-yibDp2-GFPuv

   Plasmid

   #139083

   Purpose

   Low phosphate inducible promoter yibDp2 for GFP expression

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

   Insert

   yibDp2-GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMPTY::sgRNA2

   Plasmid

   #165459

   Purpose

   Escherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria

   Depositor

   Inigo Lasa

   Article

   Dorado-Morales et al mBio. 2021 Feb 23;12(1). pii: mBio.03094

   Insert

   sgRNA2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   SP01

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRT802

   Plasmid

   #68113

   Purpose

   Integration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.

   Depositor

   Maggie Smith

   Article

   Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

   Inserts

   aac3(IV)
   aph11
   oriT
   attP
   int
   

   Use

   Streptomyces integrating vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p006-GFP-Logic-AND

   Plasmid

   #112237

   Purpose

   GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Inserts

   GFP under control of doubly-repressible promoter
   GFP
   araC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   AND-gate and pLacI

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas3

   Plasmid

   #229538

   Purpose

   pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Insert

   Cas3
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLS.491

   Plasmid

   #184956

   Purpose

   Test effect of a1/a2 length on editing rpoB using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   rpoB donor T1534C

   Promoter

   T7/lac

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJB042 (FtsZ-mEos2)

   Plasmid

   #49764

   Purpose

   Inducible expression of FtsZ-mEos2 in bacteria

   Depositor

   Jie Xiao

   Article

   Buss et al Mol Microbiol. 2013 Sep;89(6):1099-120.

   Insert

   FtsZ-mEos2
   

   Use

   Tags

   mEos2

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3xFlag-Dam

   Plasmid

   #121909

   Purpose

   Expresses E. coli Dam methyltransferase containing an N-ternimal 3xFlag tag

   Depositor

   Douglas Feldman

   Article

   Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

   Insert

   Dam human codon optimized (dam E. coli)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)

   Plasmid

   #199763

   Purpose

   Elongation reporter construct to quantify elongation of LacZ

   Depositor

   Brian Zid

   Article

   Guzikowski et al RNA Biol. 2022;19(1):636-649. doi: 10.10

   Insert

   LacZ-nLucPEST
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TetO7

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. ABE8r-R153del

    Plasmid

    #208296

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-R153del-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-subtilase A

    Plasmid

    #35948

    Depositor

    Zhaoyang Feng

    Article

    Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

    Insert

    Subtilase A (subA E. Coli)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pSCL.139

    Plasmid

    #184994

    Purpose

    Express Cas9-P2A-Eco1RT in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    SpCas9-P2A-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Human codon optimized RT

    Promoter

    TetOn-3g

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET FLII12Pglu-700uDelta6

    Plasmid

    #13568

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    FLIPglu F16A Y12S-ECFP-D13R, 6aa linker
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in mglB

    Promoter

    Available since

    Feb. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXD70LacZ2-Pdadh-LacZ

    Plasmid

    #191618

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. P628_SpCas9-RecA

    Plasmid

    #87263

    Purpose

    Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CBh

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-mCherry-2xEcMTS

    Plasmid

    #133624

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherry-2xEcMTS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF-ENTR-LacZ (710-1)

    Plasmid

    #17430

    Purpose

    Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    LacZ
    

    Use

    Entry vector

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLPE1

    Plasmid

    #209981

    Purpose

    Contains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    E. coli shuttle part (pMB1_ampR_B0017)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pLPE2

    Plasmid

    #209982

    Purpose

    Contains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    E. coli shuttle part (ampR_B0017)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pLPE3

    Plasmid

    #209985

    Purpose

    Contains Level 0* Part: E. coli shuttle part (pMB1_cat) for the construction of Level 2 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    E. coli shuttle part (pMB1_cat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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