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Plasmid#129014PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporterDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0650
Plasmid#129011PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporterDepositorInsert5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0648
Plasmid#129009PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effectsDepositorInsert5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0651
Plasmid#129012PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporterDepositorInsert5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
K8.1Pr-pGL4.16-OriLyt
Plasmid#131038PurposeFirefly Luciferase reporter for K8.1 promoter from KSHV with the lytic origin of replicationDepositorInsertKSHV Lytic Origin of replication
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGS1112
Plasmid#63784PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by URA3 selection markerDepositorInserteSapphire-Strep-tag II followed by URA3
UseE. coli cloning vectorTagsSapphire-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-103 Y32F Y96F
Plasmid#47092DepositorInsertAL-103 Y32F Y96F (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationY32F Y96FPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/ARE
Plasmid#62857PurposeSplicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationdestabilizing sequences (DS) added to the C termi…PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC
Plasmid#135465PurposeMembrane-anchored TF used for yeast directed evolution of TEV proteaseDepositorInsertSTE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16
UseTagsBFP, flagExpressionYeastMutationPromoterTDH3Available sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hChATp-tdTomato
Plasmid#197564PurposeExpresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragmentDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterhuman ChAT promoterAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_NP_EBOV
Plasmid#103049PurposeEncodes Ebola virus NP gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertNP gene of Ebola virus (NP Zaire ebolavirus, Mayinga)
UseTagsExpressionMammalianMutationsilent mutation (T to C) at position 928 of NP ge…PromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV:cTNT::Luciferase
Plasmid#69915PurposeUsed to package AAV9:cTNT::luciferase. Specifically transduce cardiomyocytes.DepositorInsertFirefly luciferase
UseAAVTagsExpressionMutationPromoterChicken cardiac troponin TAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHDR2_DAZL-T2A-mGreenLantern-PuroTK
Plasmid#222917PurposeHomology directed repair template for knocking in mGreenLantern reporter to DAZL.DepositorInsertmGreenLantern
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
EFS-Vector
Plasmid#109395PurposeAAV-CRISPR knockout plasmid for direct in vivo genome editingDepositorTypeEmpty backboneUseAAV, CRISPR, Mouse Targeting, and Synthetic Biolo…TagsExpressionMammalianMutationPromoterEFs-NSAvailable sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTTC840 - pAAV 5xATF6 secNLuc
Plasmid#82497PurposeLongitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivoDepositorInsertsecNLuc
UseAAVTagsExpressionMammalianMutationPromoter5x(ATF6 binding site) c-Fos minimal promoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
sfTq2ox-PBP5
Plasmid#117960PurposePeriplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)DepositorInsertsfTq2ox-PBP5 (dacA )
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHDR-USR-SpCas9
Plasmid#208829PurposeHDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
tet_pLKO.1_puro_shLuc
Plasmid#136587PurposeExpresses an inducible short hairpin targeting firefly luciferase sequenceDepositorInsertshLuc
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_3E5E_eGFP
Plasmid#103054PurposeEncodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoterDepositorInsertEbola virus (ebolavirus Zaire, Mayinga) minigenome
UseTags3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…ExpressionMammalianMutationPromoterCAGAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pL1M-pMtRC-MtLAP1-Adh-R1
Plasmid#170790PurposeA Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic StudiesDepositorInsertMtLAP1 (LOC11412013 Medicago truncatula)
UseTagsExpressionBacterial and PlantMutationPromoterMedtr4g059670(MtRC)Available sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only