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pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

Plasmid

#45578

Purpose

Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

coY2 Ani

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pSELt_S7.A

Plasmid

#125835

Purpose

Negative selection plasmid for evolution of an amber stop codon suppression tRNA

Depositor

Andrew Ellington

Article

Maranhao et al ACS Synth Biol. 2017 Jan 20;6(1):108-119

Insert

ACP-2xAmb-pheS

Use

Synthetic Biology

Expression

Bacterial

Promoter

pr.trpL*

Available Since

May 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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CpcB-Solanum-lycopersicumPHLS_CpcA

Plasmid

#74003

Purpose

Expresses the Solanum lycopersicum PHLS as a fusion with the CpcB protein plus the NPPS and CmR cassete in Synechocystis

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

Inserts

PHLS and CmR
Neryl diphosphate synthase from Solanum lycopersicum

Promoter

cpc

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pYFP-lacY-.008

Plasmid

#140140

Purpose

Evaluating the impact of feedback and low sensor concentration on information transmission of a genetic sensor.

Depositor

Drew Tack

Article

Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

Insert

lacY (lacY )

Expression

Bacterial

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV Sbf-Sal linker

Plasmid

#50434

Purpose

Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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pPV230.13

Plasmid

#14902

Depositor

James Lok

Article

Li et al Int J Parasitol. 2006 May 31. 36(6):671-

Insert

Strongyloides stercoralis transitional endoplasmic reticulum ATPase promoter region

Expression

Worm

Mutation

Ss era-1 promoter region as a HindIII-PstI fragme…

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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WDR34

Plasmid

#132526

Purpose

Human WDR34, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

WDR34 (WDR34 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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TCTEX1D2

Plasmid

#132529

Purpose

Human TCTEC-1D2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

TXCTEX1D2 (TCTEX1D2 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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WDR60

Plasmid

#132525

Purpose

Human WDR60, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

WDR60 (WDR60 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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px330 EQR

Plasmid

#101733

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pML2(−)WF

Plasmid

#108337

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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6his R.3-AR GUSpET28a+/pLysS

Plasmid

#61157

Purpose

N-terminal His tagged round 3 aldehyde-resistant GUS expression vector

Depositor

Andrew Ellington, Ichiro Matsumura

Article

Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

Insert

GUSAR

Tags

6x His

Expression

Bacterial

Mutation

N66D, D151N, A219V, I396T, T480A, Q498R, D508E an…

Promoter

T7

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pML2(+)BR

Plasmid

#108336

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)WF

Plasmid

#108333

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)BF

Plasmid

#108334

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)WR

Plasmid

#108335

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)BF

Plasmid

#108338

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)WR

Plasmid

#108339

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)BR

Plasmid

#108340

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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PLL7.0 tgRFP LOV

Plasmid

#114463

Purpose

Mammalian expression of tgRFP labelled LOV2

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

LOV2

Tags

tgRFP

Expression

Mammalian

Mutation

G401A(G528A), N411E(N538E) and V289I (V416I)

Promoter

CMV

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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