We narrowed to 7,454 results for: ski

1. pcDNA5-FRT-TO-FH-Nsp7

   Plasmid

   #157689

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp7 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pGEX tensin-SH2

   Plasmid

   #46525

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   tensin (TNS1 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1451-1588

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5-FRT-TO-Nsp15-HF

   Plasmid

   #157722

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp15-HF (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. LK4-Ets2

   Plasmid

   #44606

   Depositor

   Robert Oshima

   Article

   Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

   Insert

   Ets2 (Ets2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human Beta-Actin

   Available since

   May 13, 2013

   Availability

   Academic Institutions and Nonprofits only

5. CRY-tTA (B702)

   Plasmid

   #92032

   Purpose

   Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   tTA2 (TetR fused to VP64)

   Expression

   Mammalian

   Mutation

   NLS on CRY2 is mutated

   Promoter

   CMV

   Available since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FSW-HRP-V5-MDGA1

   Plasmid

   #82541

   Purpose

   Lentiviral vector for expressing HRP-MDGA1 in neurons

   Depositor

   Alice Ting

   Article

   Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

   Insert

   MDGA1 (MDGA1 Human)
   

   Use

   Lentiviral

   Tags

   HRP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX Zap70(N)-SH2

   Plasmid

   #46534

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Zap70(N) (ZAP70 Human)
   

   Use

   Tags

   GST and Thrombin site

   Expression

   Bacterial

   Mutation

   contains amino acids 1-116

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX Jak2-SH2

   Plasmid

   #46448

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Jak2 (JAK2 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 394-497

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX Grb14-SH2

   Plasmid

   #46439

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Grb14 (GRB14 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 427-540

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3 HRPT2 227X

    Plasmid

    #11050

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 227X (CDC73 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncation mutant with a stop codon introduced at…

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

11. mCherry-FKBP-TPD53

    Plasmid

    #172457

    Purpose

    Expression of Tumor Protein D52 like 1 (TPD53/TPD52L1) with N-terminal mCherry-FKBP tag

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD53 (TPD52L1 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV HA hRb delta CDK + S230

    Plasmid

    #58907

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S230 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCMV HA hRB delta CDK + T252

    Plasmid

    #58910

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T252 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

14. 1029-AIDx-CP

    Plasmid

    #135341

    Purpose

    Expression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    CP 1029 nSpyCas9-hAIDx
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ArpC2_pLib

    Plasmid

    #173679

    Purpose

    ArpC2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC2 (ARPC2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPN235

    Plasmid

    #91592

    Purpose

    Express sgRNA targeting human CYFIP1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPN234

    Plasmid

    #91591

    Purpose

    Express sgRNA targeting human CYFIP1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti CRISPR V2 sgShmt2_1

    Plasmid

    #106314

    Purpose

    Express Cas9 and sgRNA targeting mShmt2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT2 (Shmt1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAC4 Grb14-PIRSH2

    Plasmid

    #46438

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Grb14 (GRB14 Human)
    

    Use

    Tags

    AviTag, GST, and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 341-540

    Promoter

    Trc

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWZLblasti-HA-MEK5

    Plasmid

    #53167

    Purpose

    Expresses HA tagged human MEK5 from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK5 (MAP2K5 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only