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pcDNA3.3-hALDOA

Plasmid

#246126

Purpose

For transient transfection of cells for expression of human ALDOA

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hALDOB

Plasmid

#246127

Purpose

For transient transfection of cells for expression of human ALDOB

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOB (ALDOB Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_2xRecEnhancedEdited_1xBoxB_EYFP

Plasmid

#250546

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin without two editible stop codons

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_2xRecEnhancedEdited_1xBoxB_EYFP

Use

Synthetic Biology

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_2xRecEnhancedSTOP_1xBoxB_EYFP

Plasmid

#250545

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin with two editible stop codon

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_2xRecEnhancedSTOP_1xBoxB_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_1xRecEnhancedSTOP_1xBoxB_EYFP

Plasmid

#250543

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin with one editible stop codon

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_1xRecEnhancedSTOP_1xBoxB_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_1xRecEnhancedEDITED_1xBoxB_EYFP

Plasmid

#250544

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin without an editible stop codon

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_1xRecEnhancedEDITED_1xBoxB_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pTarget-entry

Plasmid

#220996

Purpose

Prokaryotic expression of mRFP and GFP. Target sequences are inserted in place of the RFP gene through AatII/KpnI restriction ligation

Depositor

John van der Oost

Article

Swartjes et al iScience. 2025 Nov 12;28(12):114033. doi

Insert

mRFP, GFP

Expression

Bacterial

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRpuro-sgSEC24D

Plasmid

#231565

Purpose

pLentiCRISPR expression vector for Cas9 and gRNA targeting human SEC24D (Puromycin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

sgSEC24D (SEC24D Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF-1a

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pJH4040

Plasmid

#229803

Purpose

Pgpc-1 wCherry unc-54 3' UTR C.elegans neuron and other structures expression of RFP

Depositor

Mei Zhen

Article

Lin et al Sci Adv. 2023 Mar;9(9):eade1249. doi: 10

Insert

Pgpc-1 wCherry unc-54 3' UTR

Tags

RFP

Expression

Worm

Promoter

Pgpc-1

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2 puro mGSDME gRNA2

Plasmid

#223522

Purpose

Knocking out mGSDME in mouse cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2 puro mGSDME gRNA1

Plasmid

#223521

Purpose

Knocking out mGSDME in mouse cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pNMTMA_MBP_Z_Cav1_H6_Periplasmic_mScarlet

Plasmid

#223703

Purpose

To produce caveospheres with periplasmically encapsulated mScarlet

Depositor

Rob Parton

Article

Luong et al ACS Nano. 2025 Dec 23;19(50):42079-42096

Insert

MBP_Z_Cav1_H6_Periplasmic_mScarlet

Expression

Bacterial

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pRetro-UMI

Plasmid

#222686

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 EGFP sgRNA4

Plasmid

#164934

Purpose

Expresses EGFP sgRNA4 in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

sgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)

Use

Lentiviral

Promoter

EFS promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAdEasy-hALDOA K230A

Plasmid

#210814

Purpose

To block the formation of the initial Schiff base with FBP

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Adenoviral

Tags

HA

Mutation

residues 230 of ALDOA mutated to alanine

Promoter

CMV

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ262F-ABE8e

Plasmid

#199179

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e-HF mediated A-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Plant Physiol. 2023 Aug 31;193(1):291-30

Insert

ABE8e(V106W)-zSpCas9(D10A)

Use

CRISPR; Gateway compatible abe8e(v106w)-zspcas9(d…

Tags

NLS

Expression

Plant

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Mta1+exon minimal CMV pCDNA5

Plasmid

#212070

Purpose

LR vector for integration of Mta1+exon(12nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Mta1+exon(12nt) (Mta1 Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Gatad2a+exon minimal CMV pCDNA5

Plasmid

#212050

Purpose

LR vector for integration of Gatad2a+exon(75nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Gatad2a+exon(75nt) (Gatad2a Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-BirA-FLAG Chtop-exon minimal CMV pCDNA5

Plasmid

#212090

Purpose

LR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Chtop-exon (Chtop Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Tcf12+exon minimal CMV pCDNA5

Plasmid

#212077

Purpose

LR vector for integration of Tcf12+exon(72nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Tcf12+exon(72nt) (Tcf12 Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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