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pCAG MCP-VP64

Plasmid

#92357

Purpose

CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

MCP-VP64

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flightless-1-LRR-HA

Plasmid

#21151

Depositor

Junying Yuan

Publication

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Insert

pcDNA3-Fli-Lrr-HA

Tags

HA

Expression

Mammalian

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pLNC Wnt-1

Plasmid

#17991

Depositor

Jan Kitajewski

Publication

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-1 (Wnt1 Mouse)

Use

Retroviral

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
STAGR_gRNAScaffold_mU6

Plasmid

#102844

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Publication

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert gRNAScaffold_mU6

Use

Pcr template for stagr inserts

Promoter

mU6

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCI Cas9 H2B-RFP

Plasmid

#92397

Purpose

CAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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LRP6N-IgG-pCS2

Plasmid

#27279

Depositor

Xi He

Publication

Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Tags

IgG Fc

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLIA-E p27 (CC#620)

Plasmid

#15191

Depositor

Connie Cepko

Publication

Dyer et al J Neurosci. 2001 Jun 15. 21(12):4259-71.

Insert

p27 (Cdkn1b Mouse)

Use

Retroviral

Tags

Flag and His

Expression

Mammalian

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-HseIF4E

Plasmid

#79437

Purpose

Expresses EGFP-tagged HseIF4E in Mammalian cells

Depositor

Elisa Izaurralde

Publication

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

HseIF4E (EIF4E Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMIR-21 Reporter

Plasmid

#71870

Purpose

Mammalian expression vector for the analysis of miR-21 activity

Depositor

Mien-Chie Hung

Publication

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-21

Tags

firefly luciferase

Expression

Mammalian

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pIRESpuro2-UBXD1-mCherry

Plasmid

#31835

Depositor

Hemmo Meyer

Publication

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

UBXD1 (UBXN6 Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
3486 pBABE Bid p22 GFP

Plasmid

#8777

Depositor

Stanley Korsmeyer

Publication

Zha et al Science 2000 Dec 1;290(5497):1761-5.

Insert

Bid p22 (Bid Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pQF2wWB

Plasmid

#61313

Purpose

Enhancer trap version of QF2w, analogous to pGAWB

Depositor

Christopher Potter

Publication

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF2w

Expression

Insect

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pQF2WB

Plasmid

#61312

Purpose

Enhancer trap version of QF2, analogous to pGAWB

Depositor

Christopher Potter

Publication

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF2

Expression

Insect

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET14b PHAS-I F113A

Plasmid

#15680

Depositor

John Lawrence

Publication

Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

Insert

PHAS-I F113A (Eif4ebp1 Rat)

Tags

6xHis

Expression

Bacterial

Mutation

F113A mutation.

Available since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only
1113 pSG5L HA PTEN D3

Plasmid

#10752

Depositor

William Sellers

Publication

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

D3: S380D, T382D, T383D

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pAW91.dCas9

Plasmid

#104372

Purpose

Expresses dCas9

Depositor

Richard Young

Publication

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

dCas9

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1(+)-OTI-TCRA_p2_TCRB

Plasmid

#131033

Purpose

Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptide

Depositor

Jeff Hammerbacher

Publication

Aksoy et al bioRxiv

Inserts

TCRA (Tcra Mouse)
TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV/T7

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET30-ARH3-His-Sumo-HA

Plasmid

#111578

Purpose

Expresses ARH3 (amino acids 14-363) in E. coli

Depositor

Thomas Muir

Publication

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

ARH3 (ADPRS Human)

Tags

6xHis-Sumo and HA

Expression

Bacterial

Mutation

deleted amino acids 1-13

Promoter

T7

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP

Plasmid

#165830

Purpose

EGFP green fluorescent protein CDS

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

EGFP

Expression

Insect

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pDSG312

Plasmid

#115613

Purpose

surface-displayed Nb2, Arabinose-inducible; medium-copy

Depositor

Ingmar Riedel-Kruse

Publication

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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