Addgene

Showing: 2351 - 2360 of 14497 results

Results per page:

Sort results by:

pET28a-OuIAD(C500S)

Plasmid

#226970

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) C500S variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(C500S)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

C500S

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-OuIAD(G853A)

Plasmid

#226969

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) G853A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(G853A)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

G853A

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-NEDD4L_W4-HECT

Plasmid

#187710

Purpose

Express his-tagged NEDD4L W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 543-975 (NEDD4L Human)

Use

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-542

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-NEDD4L_W3-W4-HECT_C874only

Plasmid

#187711

Purpose

Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 with mutations (NEDD4L Human)

Use

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-491; mutations C702A, C776…

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanIV-Streptomyces

Plasmid

#226250

Purpose

Expresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIV-Streptomyces)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-a(+)-6xHis-mScarlet-I(+5)

Plasmid

#219418

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

mScarlet-I(+5)

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

D125K, D186K, E216K, E238K

Promoter

T7

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b(+)_gp1(A11V)

Plasmid

#225131

Purpose

Phage GIL01 protein gp1 (A11V) mutant with a single nucleotide substitution (C32T)

Depositor

Matej Butala

Insert

gp1 (A11V)

Use

Tags

His-tag

Expression

Bacterial

Mutation

Alanine 11 to Valine

Promoter

T7 promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-mPTER-H230F

Plasmid

#222195

Purpose

Bacterial expression of mouse PTER-H230F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H230F

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-mPTER-H26F

Plasmid

#222192

Purpose

Bacterial expression of mouse PTER-H26F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H26F

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-dCas9-FCPF

Plasmid

#220133

Purpose

Expresses dCas9-FCPF in E.coli

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

dCas9-FCPF (cas9 S. pyogenes, Synthetic)

Use

Tags

6xHis

Expression

Bacterial

Mutation

FCPF sequence inserted on C-terminal

Promoter

T7

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge