We narrowed to 7,063 results for: poly
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Plasmid#169135PurposeE. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertCoh7
Tags3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…ExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA269
Plasmid#216073PurposeFragmid fragment: (CROPseq) WPRE; 3'LTR; guide expression bGHpolyADepositorHas ServiceCloning Grade DNAInsertWPRE_v1; U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]; 3'LTR; bGHpolyA_v1
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo(K48R)-GFP
Plasmid#212793PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeastDepositorInsertHis6-CUbo(K48R)-GFP
UseIntegrative vectorExpressionYeastMutationCUb(K48R)PromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo(K63R)-GFP
Plasmid#212794PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeastDepositorInsertHis6-CUbo(K63R)-GFP
UseIntegrative vectorExpressionYeastMutationCUb(K63R)PromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(48) aa24-150-VSV
Plasmid#212747PurposeConstitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)DepositorInsertE3(48) aa24-150
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationCue1 ΔU7BDPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-myc-E3(1) C885A-CUbo-VSV
Plasmid#212738PurposeConsitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)DepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-NLS-VSV and mycExpressionYeastMutationHOIP(C885A)PromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
Plasmid#212805PurposeConstitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cellsDepositorInsertNUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
ExpressionMammalianAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_aa 321-843 REV1
Plasmid#201587PurposeExpresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cellsDepositorInsertDNA repair protein REV1 (REV1 Human)
TagsHAExpressionMammalianMutationExpresses amino acids 321-843 of DNA repair prote…Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)
Plasmid#201545PurposeExpresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23BDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
TagsFLAGExpressionMammalianMutationLacks N-terminal residues 1-79Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)
Plasmid#201546PurposeExpresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23BDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
TagsFLAGExpressionMammalianMutationLacks residues 188-228Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)
Plasmid#201548PurposeExpresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23BDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
TagsFLAGExpressionMammalianMutationLacks C-terminal residues 364-409Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTdelta5cys
Plasmid#207465PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variantDepositorInsertTdTdelta5cys (Dntt Mouse)
Tags10xHisExpressionBacterialMutationCys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…Available SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3UTR
Plasmid#202550PurposeReady for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation mediaDepositorTypeEmpty backboneTagsATG initiation codon followed by an IGKV3 signal …ExpressionMammalianPromoterCMVAvailable SinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hSTK16-Flag
Plasmid#180403Purposelentiviral transduction of human STK16 (with a C-terminal Flag tag) geneDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
W118-1_Flag-hSRPK3
Plasmid#180404Purposelentiviral transduction of human SRPK3 (with an N-terminal Flag tag) geneDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shTgm5-1
Plasmid#180394PurposeProducing AAV that encodes mouse Tgm5 shRNA-1 with miR-E backboneDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shTgm5-2
Plasmid#180395PurposeProducing AAV that encodes mouse Tgm5 shRNA-2 with miR-E backboneDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shTgm5-3
Plasmid#180396PurposeProducing AAV that encodes mouse Tgm5 shRNA-3 with miR-E backboneDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔ(N+HAT)
Plasmid#182841PurposeAn EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP (w Fly)
TagsFLAG tagExpressionInsectMutationdletion of residues 1566-2142 from CBP1476-3222PromoterMTAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(K206A/K244A)
Plasmid#177135PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A) with a TEV protease site located between the GST tag and PolB(K206A/K244A)DepositorInsertPolB
TagsGSTExpressionBacterialPromoterTacAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only