We narrowed to 19,534 results for: ACE

1. pKDsgRNA-ompT

   Plasmid

   #102291

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ompT.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   ompT gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKDsgRNA-srlA

   Plasmid

   #89965

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting srlA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   srlA gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKDsgRNA-rbsK

   Plasmid

   #89963

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rbsK.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   rbsK gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKDsgRNA-yhhT

   Plasmid

   #89958

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting yhhT.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   yhhT gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKDsgRNA-torZ

   Plasmid

   #89956

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   torZ gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKDsgRNA-arsB

   Plasmid

   #89952

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   arsB gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BC-A1-016

   Plasmid

   #78704

   Purpose

   A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BC-A1-015

   Plasmid

   #78703

   Purpose

   A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BC-A1-010

   Plasmid

   #78697

   Purpose

   A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.

   Depositor

   Brian Chow

   Article

   Magaraci et al ACS Synth Biol. 2016 Apr 25.

   Insert

   BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BC-A1-008

    Plasmid

    #78695

    Purpose

    A weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BC-A1-007

    Plasmid

    #78694

    Purpose

    A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVH004

    Plasmid

    #80401

    Purpose

    Contains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    CusR (cusR )
    AraC (araC )
    Anti-scaffold
    

    Use

    Synthetic Biology

    Tags

    C-terminal LZx domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVH015

    Plasmid

    #80398

    Purpose

    Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    Anti-scaffold-ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal 3xFLAG tag and C-terminal LZx domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBP-J23108

    Plasmid

    #72964

    Purpose

    Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23108 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBP-J23114

    Plasmid

    #72965

    Purpose

    Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23114 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBP-T7_RBS

    Plasmid

    #72978

    Purpose

    T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    T7+RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBP-Strep(II)

    Plasmid

    #72984

    Purpose

    Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Strep(II) N-terminal tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTU1-C-RFP

    Plasmid

    #72941

    Purpose

    Level 1 Destination vector - Position C

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 1 - C site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Beta-lactamase - G717C (nucleotide) - silent muta…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBP-lacZ

    Plasmid

    #72948

    Purpose

    Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 0 - Bioparts
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTU2-A-lacZ

    Plasmid

    #72950

    Purpose

    Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - A site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge