We narrowed to 5,112 results for: codon optimized

1. p3E-DrCav1

   Plasmid

   #194290

   Purpose

   Multisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRT

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   caveolin1 (cav1.S Frog)
   

   Use

   Multisite gateway entry vector

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLSB-NAT

   Plasmid

   #166698

   Purpose

   Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Expression

   Yeast

   Promoter

   adh15 promoter and tRNA promoter

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLSB-KAN

   Plasmid

   #166700

   Purpose

   Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Expression

   Yeast

   Promoter

   adh15 promoter and tRNA Ser promoter

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLSB-HYG

   Plasmid

   #166699

   Purpose

   Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Expression

   Yeast

   Promoter

   adh15 promoter and tRNA promoter

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. EBA175-bio

   Plasmid

   #47743

   Purpose

   Expresses enzymatically monobiotinylated full-length EBA175 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised EBA175
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. RH2b-bio

   Plasmid

   #47778

   Purpose

   Expresses enzymatically monobiotinylated partial RH2b ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised RH2b (partial)
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIB-HIS-TEV-CKS1

   Plasmid

   #177013

   Purpose

   Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   CKS1 (CKS1B Human, Synthetic)
   

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Mutation

   codon-optimised for insect cell expression

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. RH4-bio

   Plasmid

   #47779

   Purpose

   Expresses enzymatically monobiotinylated partial RH4 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised RH4 (partial)
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. EBA181-bio

   Plasmid

   #47744

   Purpose

   Expresses enzymatically monobiotinylated full-length EBA181 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised EBA181
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Pf92-bio

    Plasmid

    #47728

    Purpose

    Expresses enzymatically monobiotinylated full-length Pf92 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised Pf92
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. RON6-bio

    Plasmid

    #47785

    Purpose

    Expresses enzymatically monobiotinylated full-length RON6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RON6
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin replaced…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221_SLC25A51

    Plasmid

    #132019

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A51 (SLC25A51 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCMV-PE7-P2A-hMLH1dn

    Plasmid

    #222997

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-hMLH1dn
    

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Deletion of residues 754-756 in MLH1 as described…

    Promoter

    CMV

    Available Since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. LSL-Cas9-Rosa26TV

    Plasmid

    #61408

    Purpose

    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    Cas9
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    3xFLAG and P2A

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CAG

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3TS-RfxCas13d-HA

    Plasmid

    #141320

    Purpose

    Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

    Depositor

    Ariel Bazzini, Miguel Angel Moreno-Mateos

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d
    

    Use

    CRISPR

    Tags

    HA

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYeDP60-hATP13A2-E343A

    Plasmid

    #171378

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Tags

    BAD tag

    Expression

    Yeast

    Mutation

    E343A

    Promoter

    URA3

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221_MFSD8

    Plasmid

    #131882

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    MFSD8 (MFSD8 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pSCAR_Cas9-blast_GFP

    Plasmid

    #162074

    Purpose

    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Inserts

    Cas9-2a-blasticidin S deaminase
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EF1a and sv40

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT3TS-RfxCas13d-NLS-HA

    Plasmid

    #141321

    Purpose

    Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

    Depositor

    Ariel Bazzini, Miguel Angel Moreno-Mateos

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d (NLS-RfxCas13d-NLS)
    

    Use

    CRISPR

    Tags

    HA and NLS

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223 SARS-CoV-2 NSP6

    Plasmid

    #141260

    Purpose

    Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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