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pNB691

Plasmid

#60135

Purpose

Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

RdLuc

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Promoter

MET17pr

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNB753

Plasmid

#60148

Purpose

Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

GrLuc

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Promoter

LEU1pr

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSG5-p85α-Δbcr

Plasmid

#39500

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 Δbcr (PIK3R1 Bovine)

Use

Eukaryotic

Mutation

Δbcr = deletion of AA134-302

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pLXSN-p85α_wt

Plasmid

#39501

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 (PIK3R1 Bovine)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pUC19_PHF19_KO_Puro_donor

Plasmid

#246401

Purpose

PHF19 KO donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Expression

Mammalian

Mutation

KO

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_MTF2_KO_Puro_donor

Plasmid

#246393

Purpose

MTF2 KO donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

MTF2 (MTF2 Human)

Expression

Mammalian

Mutation

KO

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_MTF2_HTC3xF_BSD_donor

Plasmid

#246391

Purpose

MTF2 donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

MTF2 (MTF2 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF1_HTC3xF_Puro_donor

Plasmid

#246395

Purpose

PHF1 donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF1_KO_Puro_donor

Plasmid

#246397

Purpose

PHF1 KO donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Expression

Mammalian

Mutation

KO

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF19_HTC3xF_Puro_donor

Plasmid

#246399

Purpose

PHF19 donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_MTF2_sgRNA

Plasmid

#246402

Purpose

Cas9/sgRNA expression plasmid targeting MTF2

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

MTF2 (MTF2 Human)

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pPBneo-UbC-mCherry

Plasmid

#228759

Purpose

Encoding mCherry.

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

mCherry

Expression

Mammalian

Promoter

UbC promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSGEP-shLuciferase_3

Plasmid

#239617

Purpose

Control non-targeting shRNA

Depositor

Reza Kalhor

Article

Handler et al Cell Rep. 2025 Dec 15;44(12):116701. doi

Insert

shLuciferase_3

Use

Lentiviral

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR221-Illusia

Plasmid

#215442

Purpose

Gateway entry vector with Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia

Use

Gateway entry vector

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPB.DEST-Illusia(YYFF)

Plasmid

#215445

Purpose

Piggybac expression vector with inactive phospho-mutant (YYFF) of Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia(YYFF)

Use

Transposon-based stable expression

Expression

Mammalian

Promoter

CAG

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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dimerTomato-wGBD

Plasmid

#129635

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

Cdc42 binding domain of N-WASP

Expression

Mammalian

Promoter

CMVdel

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

Plasmid

#217478

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1s

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

Plasmid

#217479

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1b

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMamCol1

Plasmid

#215184

Purpose

Allows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coli

Depositor

Krishna Kishore Inampudi

Insert

tsPurple

Use

AAV, CRISPR, and Synthetic Biology ; Recombinant …

Tags

SNAC-StrepII tag

Expression

Bacterial and Mammalian

Promoter

TetON 3G Bidirectional for Mammalian and Trc with…

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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MCP-YPet

Plasmid

#215747

Purpose

Expresses MS2 coat protein (MCP) fused to YPet

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

YPet

Expression

Mammalian

Promoter

EF1a

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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