Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 13,056 results for: BASE;

Showing: 4721 - 4740 of 13056 results

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EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235271

Purpose

ComMAND closed-loop circuit regulating mRuby2, EF1a

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF4)-TS.FF4x1-bGH

Plasmid

#235283

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF4

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF5)-TS.FF5x1-bGH

Plasmid

#235261

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miRE-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-tagBFP-bGH

Plasmid

#235257

Purpose

tagBFP expression to serve as a "filler" plasmid for transfections

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

tagBFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

Silent mutations to remove BsaI sites in tagBFP

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pQdCas9-sggfp

Plasmid

#236184

Purpose

The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the gfp gene.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

Quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene

Promoter

Quorum sensing promoter

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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Reckleen_3plus_sgRNA_ptet_pos4

Plasmid

#233459

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 4, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 4, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5

Plasmid

#233460

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5a

Plasmid

#233461

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5a1

Plasmid

#233462

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a1, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a1, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5a2

Plasmid

#233463

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a2, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a2, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Reckleen_3plus_sgRNA_ptet_pos5b

Plasmid

#233464

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Reckleen_3plus_sgRNA_ptet_pos5b1

Plasmid

#233465

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b1, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b1, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5b2

Plasmid

#233466

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b2, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b2, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pQdCas12a.sggfp(A)

Plasmid

#236038

Purpose

The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pQdCas12a.sggfp(B)

Plasmid

#236040

Purpose

The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His-E1GFP

Plasmid

#233885

Purpose

Bacterial Expression of recombinant His-tagged E1GFP

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

E1GFP

Tags

His

Expression

Bacterial

Mutation

T65S and T203Y compared to EGFP

Promoter

T7 promoter

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-HIS3

Plasmid

#229230

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt

Plasmid

#233240

Purpose

Parallel coiled-coil with (GGS)3 linker to prone condensation

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-clover-GCN4-(GGS)3-Vt

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Vh-clover-GCN4-(GGS)12-Vt

Plasmid

#233241

Purpose

Parallel coiled-coil with (GGS)12 linker to prevent condensation

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-clover-GCN4-(GGS)12-Vt

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-FLAG-APEX2-NES-K.l.LEU2

Plasmid

#229229

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 4721 - 4740 of 13056 results

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