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CHI +N122D

Plasmid

#187427

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed asparagine at 130 to aspartic acid

Promoter

PH and p10

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C G83R

Plasmid

#184747

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, G83R

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-Sig10(M2)-Fc-IG

Plasmid

#183724

Purpose

Expresses the fusion protein containing the extracellular part of the human Siglec-10 A108V mutant and the Fc region of human IgG1, and GFP

Depositor

Takeshi Tsubata

Article

Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

Insert

Siglec-10

Use

Retroviral

Tags

Fc of human IgG1

Mutation

A108V

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Rock1-GBD (840-110 aa) in pEGFPN1

Plasmid

#187279

Purpose

Express EGFP-Rock1 GTPase-binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Rock1 (Rock1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

GBD (84-110aa)

Promoter

CMV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C Y78F

Plasmid

#184746

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, Y78F

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C A81T

Plasmid

#184745

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, A81T

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C K80E

Plasmid

#184744

Purpose

bacterial expression of huamn TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, K80E

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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puc19_N_tag

Plasmid

#186650

Purpose

Empty donor plasmid.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

N-terminal 3xFlag-3xHA Tag

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pST_A0A1D1UKG0_RAMVA (pBS0352)

Plasmid

#185188

Purpose

For the mammalian expression of the tardigrade protein A0A1D1UKG0_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

A0A1D1UKG0_RAMVA

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_A0A1D1UJP0_RAMVA (pBS0306)

Plasmid

#185166

Purpose

For the mammalian expression of the tardigrade protein A0A1D1UJP0_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

A0A1D1UJP0_RAMVA

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_CAHS2_RAMVA (pBS0291)

Plasmid

#185160

Purpose

For the mammalian expression of the tardigrade protein CAHS2_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2_RAMVA

Expression

Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pST_CAHS1_RAMVA (pBS0267)

Plasmid

#185150

Purpose

For the mammalian expression of the tardigrade protein CAHS1_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_RAMVA

Expression

Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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A0A1D1UNQ6_RAMVA pSecTag2(pm) (pBS0256)

Plasmid

#185145

Purpose

For the mammalian expression of the tardigrade protein A0A1D1UNQ6_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

A0A1D1UNQ6_RAMVA

Expression

Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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MAHS_RAMVA_Tanaka pSecTag (pBS0253)

Plasmid

#185142

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

MAHS_RAMVA

Expression

Mammalian

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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SAHS10_A0A1D1UJR2_RAMVA pSecTag (pBS0250)

Plasmid

#185140

Purpose

For the mammalian expression of the tardigrade protein SAHS1_A0A1D1UJR2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

SAHS1_A0A1D1UJR2

Expression

Mammalian

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C I84S

Plasmid

#184748

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, I84S

Promoter

T7

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pST_MAHS_RAMVA_mts_1-81 (pBS0918)

Plasmid

#185287

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA_mts_1-81. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

MAHS_RAMVA_mts_1-81

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_MAHS_RAMVA_mts_1-74 (pBS0917)

Plasmid

#185286

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA_mts_1-74. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

MAHS_RAMVA_mts_1-74

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-Sig10(DM)-Fc-IG

Plasmid

#183725

Purpose

Expresses the fusion protein containing the extracellular part of the human Siglec-10 R47Q A108V mutant and the Fc region of human IgG1, and GFP

Depositor

Takeshi Tsubata

Article

Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

Insert

Siglec-10

Use

Retroviral

Tags

Fc of human IgG1

Mutation

R47Q and A108V

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pJJGL003

Plasmid

#180607

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertion

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

DpbCasX with R3 loop

Use

CRISPR

Tags

10x His and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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