We narrowed to 25,093 results for: expression vector

1. pTDpelB-CTwinStrep

   Plasmid

   #45941

   Purpose

   use to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequence

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Use

   Tags

   PelB and Twin-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Trc (lactose/IPTG inducible)

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKF-P14MM2AG5h

   Plasmid

   #128254

   Purpose

   Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

   Depositor

   Gabor Balazsi

   Article

   Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

   Insert

   rtTA-Advanced::P2A::EGFP
   

   Use

   Synthetic Biology; Flp-in expression vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF-ENTR C (709-1)

   Plasmid

   #17429

   Purpose

   Gateway entry vector with EF1-a promoter, V5-His C-term, frame C 

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF-ENTR B (708-1)

   Plasmid

   #17428

   Purpose

   Gateway entry vector with EF1-a promoter, V5-His C-term, frame B

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR1A-NTAP-C (w324-1)

   Plasmid

   #17404

   Purpose

   Gateway entry vector for NTAP (N-terminal affinity purification), frame C

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   CBP and SBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR1A-NTAP-B (w323-1)

   Plasmid

   #17403

   Purpose

   Gateway entry vector for NTAP (N-terminal affinity purification), frame B

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   CBP and SBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. RCASBP-AP (E) (CC#39)

   Plasmid

   #15162

   Depositor

   Connie Cepko

   Article

   Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

   Insert

   PLAP (ALPP Human)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pENTR4-V5-2 (w234-1)

   Plasmid

   #17426

   Purpose

   Gateway entry vector for N-term V5 tag, frame B

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAJ08

   Plasmid

   #14912

   Depositor

   James Lok

   Article

   Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

   Insert

   strongyloides stercoralis actin promoter
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAJ50

    Plasmid

    #14918

    Depositor

    James Lok

    Article

    Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

    Insert

    enhanced monomeric red fluorescent protein
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAJ20

    Plasmid

    #14919

    Depositor

    James Lok

    Article

    Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

    Insert

    Strongyloides stercoralis Ribosomal protein small subunit
    

    Use

    Tags

    GFP(S65C)

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAJ09

    Plasmid

    #14913

    Depositor

    James Lok

    Article

    Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

    Insert

    strongyloides stercoralis G protein alpha subunit promoter
    

    Use

    Tags

    GFP(S65C)

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. p224 pCMV-RFP/CREM

    Plasmid

    #8401

    Purpose

    Floxed RFP inserted within the coding sequence of cre recombinase.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    RFP/CREM
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre, LOX 511 sites surrounding RFP withi…

    Promoter

    CMV

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pAM4645

    Plasmid

    #85124

    Purpose

    NSII vector expressing PkaiB-KaiBC. Nt resistant. Complements rhthyms in AMC705

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    PkaiBC-KaiBC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAJ13

    Plasmid

    #14915

    Depositor

    James Lok

    Article

    Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

    Insert

    multiple cloning site 3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. NAT-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid

    #193314

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4MYC N-terminal tagging
    

    Use

    Tags

    4MYC

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. HYG-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid

    #193316

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4MYC N-terminal tagging
    

    Use

    Tags

    4MYC

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66

    Plasmid

    #168787

    Purpose

    Donor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology; Expression in fila…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-kcnj20

    Plasmid

    #173150

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj20 (si:ch211-113j13.2 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221-kcnj12b

    Plasmid

    #173142

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj12b (kcnj12b Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge