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pLJM5-I560S-mDPYD

Plasmid

#61615

Purpose

The mouse Dpyd was used to rescue the human DPYD hairpin affect on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

Dihydropyrimidine dehydrogenase (Dpyd Mouse)

Expression

Mammalian

Mutation

Changed Isoleucine at position 560 to Serine

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-ePiv (CJT553)

Plasmid

#248215

Purpose

ePiv invertase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

ePiv invertase

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

ePiv(E257R, E285R, T290R)

Promoter

CMV and T7

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-IS621 (CJT489)

Plasmid

#248213

Purpose

bpNLS-tagged IS621 bridge recombinase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-IS621

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

CMV and T7

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-Bxb1 (EMK569)

Plasmid

#248208

Purpose

Bxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-Bxb1

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

CMV and T7

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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huCofilin K22Q SSR-mRFP

Plasmid

#252617

Purpose

Expresses shRNA resistant human cofilin with K22Q mutation that reduces nuclear uptake and serves as a cofilactin rod reporter

Depositor

James Bamburg

Article

Wiggan et al Dev Cell. 2012 Mar 13;22(3):530-43. doi:

Insert

cofilin 1 (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

K22Q; silent mutations at nt 67 to 75 in which TC…

Promoter

CMV

Available Since

March 5, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-mCherry:T2A:Bsd-EF1A-HA3xGShGLI1

Plasmid

#249270

Purpose

Expression of GLI1

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GLI1 (GLI1 Human)

Use

Lentiviral

Tags

HA + 3xGS linker

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL_GPR161-HA_V129E

Plasmid

#249269

Purpose

Expression of GPR161 V129E mutant

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GPR161 (GPR161 Human)

Tags

HA

Expression

Mammalian

Mutation

V129E

Promoter

EF-1

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pBEL2809

Plasmid

#232510

Purpose

Expression plasmid of Cys-light LetA(L94C,I383C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

[6xHis2xQH-TEV-LetA1(1-223,L94C,C124S)]-[LetA2(22…

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2820

Plasmid

#232511

Purpose

Expression plasmid of Cys-light LetA(Q180C,R380C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

[6xHis2xQH-TEV-LetA1(1-223,Q180C,C124S)]-[LetA2(2…

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16167 AU1.Flag.Ollas

Plasmid

#241479

Purpose

AU1.Flag.Ollas Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, Flag, and Ollas

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16170 AU1.FLAG.V5

Plasmid

#241480

Purpose

AU1.FLAG.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, FLAG, and V5

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16173 AU1.HA.Ollas

Plasmid

#241481

Purpose

AU1.HA.Ollas Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, HA, and Ollas

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16186 AU1.Ollas.S

Plasmid

#241484

Purpose

AU1.Ollas.S Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, Ollas, and S

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16188 AU1.OLLAS.V5

Plasmid

#241485

Purpose

AU1.OLLAS.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, OLLAS, and V5

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16175 AU1.HA.VSVG

Plasmid

#241497

Purpose

AU1.HA.VSVG Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, HA, and VSVG

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16187 AU1.OLLAS.VSVG

Plasmid

#241498

Purpose

AU1.OLLAS.VSVG Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, OLLAS, and VSVG

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16189 AU1.S.VSVG

Plasmid

#241499

Purpose

AU1.S.VSVG Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, S, and VSVG

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16191 AU1.VSVG.V5

Plasmid

#241500

Purpose

AU1.VSVG.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, V5, and VSVG

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-huCof R21Q-mRFP

Plasmid

#245964

Purpose

Expression of the weak F-actin binding R21Q cofilin-mRFP which serves as a cofilactin rod reporter without inducing rods

Depositor

James Bamburg

Article

Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

R21Q

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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huCof K22Q-mRFP

Plasmid

#245965

Purpose

Expression of the weak F-actin binding K22Q cofilin-mRFP which serves as a cofilactin rod reporter without inducing rods

Depositor

James Bamburg

Article

Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

K22Q

Promoter

CMV

Available Since

Oct. 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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