We narrowed to 12,952 results for: sequence

1. pCAG-hHDAC4

   Plasmid

   #21096

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   Histone Deacetylase 4 (HDAC4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   shRNA target sequence was mutated (mutated nucleo…

   Promoter

   CAG

   Available Since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

2. gRD21A-mRFP

   Plasmid

   #181959

   Purpose

   RD21A protein tagged with mRFP, under control of native promoter

   Depositor

   George Haughn

   Article

   Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

   Insert

   RD21A genomic sequence including 2kb upstream of the ATG
   

   Use

   Tags

   mRFP

   Expression

   Plant

   Mutation

   Promoter

   RD21A native

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIK198

   Plasmid

   #65629

   Purpose

   "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

   Depositor

   Iskra Katic

   Article

   Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. nonphosphorylatable Cenp-B-targted Aurora B FRET sensor

   Plasmid

   #45230

   Depositor

   Michael Lampson

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   nonphos Cenp-B targeted Aurora B FRET sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   t-a mutation in substrate sequence

   Promoter

   Available Since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK110

   Plasmid

   #99535

   Purpose

   contains bgl operon sequence for E. coli in vitro transcription template

   Depositor

   Robert Landick

   Article

   Kotlajich et al Elife. 2015 Jan 16;4. doi: 10.7554/eLife

   Insert

   E. coli bgl antisense region containing bglG
   

   Use

   Tags

   None

   Expression

   Mutation

   Promoter

   lambda Pr promoter used for in vitro transcriptio…

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PFD1130w-bio-His

   Plasmid

   #50823

   Purpose

   Expresses enzymatically monobiotinylated full length PFD1130w

   Depositor

   Gavin Wright

   Article

   Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

   Insert

   PFD1130w
   

   Use

   Tags

   ratCD4d3+4,biotinylation sequence, 6xHis

   Expression

   Mammalian

   Mutation

   all potential N-linked glycosylation sites (N-X-S…

   Promoter

   CMV

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHBS1388 TDP43 RRM-GFP K-R

   Plasmid

   #118796

   Purpose

   To test the effect of sequence on TDP43 RRM-GFP droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All K to R in CTD of TDP43 RRM-GFP reporter

   Promoter

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNP1 tdTomato trigger

   Plasmid

   #107358

   Purpose

   Trigger sequence for pNP1 tdTomato toehold switch

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold trigger 5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNP1 Aqua trigger

   Plasmid

   #107360

   Purpose

   Trigger sequence for pNP1 Aqua toehold switch

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold trigger 8
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TAL-L1-1.3-DEL

    Plasmid

    #103035

    Purpose

    [Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by DEL domian

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

    Insert

    TAL-L1-1.3-DEL
    

    Use

    T3 in vitro transcription for expression in mamma…

    Tags

    DEL and HA

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIneo-lambdaN-HA-HsTNRC6A_C

    Plasmid

    #146086

    Purpose

    Mammalian Expression of HsTNRC6A

    Depositor

    Elisa Izaurralde

    Article

    Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

    Insert

    HsTNRC6A (TNRC6A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation K1578E compared to the se…

    Promoter

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZF-BCB-PDZ

    Plasmid

    #127478

    Purpose

    coding sequence for synthetic zinc finger ZF-BCB-PDZ

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
    

    Use

    Cell-free transcription–translation

    Tags

    alpha 1-syntrophin PDZ domain

    Expression

    Mutation

    Promoter

    T7

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-λN-βLac

    Plasmid

    #162039

    Purpose

    Expresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)

    Depositor

    Karen Polizzi

    Article

    Moya Ramirez et al Biosens Bioelectron. 2019 Jul 15;137:199

    Insert

    λN domain of the λ phage and a β-lactamase fusion protein
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTK_Cerulean_Nanotag_70_neg_Control

    Plasmid

    #130568

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    negative oligo sequence
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTK_Cerulean_Nanotag_66_neg_Control

    Plasmid

    #130567

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    negative oligo sequence
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTK_Cerulean_Nanotag_62_neg_Control

    Plasmid

    #130565

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    negative oligo sequence
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTK_Citrine_Nanotag_35_neg_Control

    Plasmid

    #130564

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    negative oligo sequence
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTK_Citrine_Nanotag_18_neg_Control

    Plasmid

    #130560

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    negative oligo sequence
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTK_Citrine_Nanotag_19_neg_Control

    Plasmid

    #130561

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    negative oligo sequence
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTK_Citrine_Nanotag_32_neg_Control

    Plasmid

    #130563

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    negative oligo sequence
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Or45b-Gal4

    Plasmid

    #63036

    Purpose

    To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or45b, using the promoter sequences of the gene cloned 5' of Gal4

    Depositor

    Leslie Vosshall

    Article

    Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

    Insert

    Drosophila melanogaster Or45b promoter
    

    Use

    Tags

    Drosophila ADH (aa's 1-18) fused to Yeast Ga…

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

22. nPEX-LV

    Plasmid

    #12119

    Depositor

    Inder Verma

    Article

    Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

    Insert

    Matrix Metalloproteinase 2 (Mmp2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    noncatalytic fragment of MMP2 amplified via PCR, …

    Promoter

    Available Since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

23. SERA7-bio-His

    Plasmid

    #50819

    Purpose

    Expresses enzymatically monobiotinylated full length SERA7

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    SERA7
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHBS1398 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W]

    Plasmid

    #118811

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G368W in CTD of full-length TDP43

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT2-cryR;cldn5bCE1-P1Egfp

    Plasmid

    #90154

    Purpose

    contains cldn5b enhancer sequence, and cldn5b specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    cldn5bE1P1Egfp (cldn5b Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p068 pCS mTRPS1 (M)

    Plasmid

    #11040

    Depositor

    Ramesh Shivdasani

    Article

    Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

    Insert

    TRPS1 mut (Trps1 Mouse)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Two Cys residues at positions 917 and 920 in the …

    Promoter

    Available Since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

27. pre_IV_CS6_4323_v1

    Plasmid

    #126859

    Purpose

    4323 b scaffold from compatibility group 4, made by method IV, insert based on de Bruijn sequence with the an order of 7

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    IV_CS6_4323_v1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pZF-ADD-L

    Plasmid

    #127477

    Purpose

    coding sequence for synthetic zinc finger ZF-ADD-L

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
    

    Use

    Cell-free transcription–translation

    Tags

    PDZ cognate peptide ligand (VKESLV)

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. SERA6-bio-His

    Plasmid

    #50818

    Purpose

    Expresses enzymatically monobiotinylated full length SERA6

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    SERA6
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. phNFL-N98S

    Plasmid

    #132592

    Purpose

    Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutation

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    NEFL (NEFL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    293A>G; Asn98Ser

    Promoter

    CMV

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge