We narrowed to 16,033 results for: GRN

1. pU6-sp-sgRNA-RNF2_+41nick

   Plasmid

   #135958

   Purpose

   S. pyogenes sgRNA for +41 nick in RNF2

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   RNF2_+41 nicking sgRNA
   

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available Since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MiniCoopR 2xU6:gRNA, mitfa:Cas9

   Plasmid

   #118844

   Purpose

   Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Type

   Empty backbone

   Use

   CRISPR; Tol2

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

   Plasmid

   #67985

   Purpose

   Cas9 activity reporter (control) with mCherry and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKmCherryABFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pScalps eSpCas9_Zsgreen gRNA scaffold

   Plasmid

   #188449

   Purpose

   Expression of eSpCas9 tagged with Zsgreen

   Depositor

   Ajit Chande

   Article

   Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Promoter

   EF1-alpha

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330GFP-hU6-gRNA-hSpCas9

   Plasmid

   #128385

   Purpose

   GFP positive gRNA/hSpCas9 construct

   Depositor

   Budd A. Tucker

   Article

   Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

   Insert

   GFP, gRNA and hSpCas9
   

   Use

   CRISPR

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti sgRNA(MS2) mCherry

   Plasmid

   #171053

   Purpose

   Expression of Vp64-dCas9 activation system

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Insert

   sgRNA(MS2) mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9(BB)-2A-tomato SOX2-sgRNA

   Plasmid

   #196190

   Purpose

   Editing human SOX2 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   SOX2 (SOX2 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-sgRNA(lib)-puro

   Plasmid

   #119976

   Purpose

   Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Wensheng Wei

   Article

   Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6-AsCas12f-full-length-sgRNA

   Plasmid

   #204638

   Purpose

   Expression of full-length AsCas12f sgRNA in mammalian cells

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   full-length AsCas12f sgRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pegRNA-GFP>BFP_Y66H-NGG

    Plasmid

    #185480

    Purpose

    pegRNA plasmid in order to make a Y66H (TAC>CAT) conversion in the GFP gene, creating a BFP gene.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    GFP>BFP_Y66H-pegRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-sgRNA-lib 2.0

    Plasmid

    #89638

    Purpose

    sgRNA expression in mammalian cells after Gateway cloning

    Depositor

    Wensheng Wei

    Article

    Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLH-sgRNA-Sirius-8XMS2

    Plasmid

    #121939

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XMS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    human U6 promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-Basic-8x-gRNA-eGFP

    Plasmid

    #60718

    Purpose

    eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR-U6-gRNA-TRE3G-TdTomato

    Plasmid

    #192658

    Purpose

    A plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomato

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    TdTomato
    

    Expression

    Mammalian

    Promoter

    U6, TRE3G

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC103-PBNeo-U6-sgRNA-Expression

    Plasmid

    #48228

    Purpose

    sgRNA expression on PiggyBac vector with Neo-selectable marker

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT4-U6-sgRNA-CMV-EGFP

    Plasmid

    #239587

    Purpose

    Sleeping-beauty based EV for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Type

    Empty backbone

    Use

    Sleeping beauty transposone

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR8-attL3-pegRNA-attL2

    Plasmid

    #213052

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSB700 lenti gRNA Puro

    Plasmid

    #167911

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-sgRNA-CBh-eGFP

    Plasmid

    #206885

    Purpose

    cloning backbone for sgRNA using OptScf2

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

    Type

    Empty backbone

    Promoter

    U6

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ3538 eforRed-targeting sgRNA

    Plasmid

    #239455

    Purpose

    guide for eforRed

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    guide targeting eforRed
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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