We narrowed to 80,523 results for: Mycs;

1. pLV-dCas9-p300-P2A-PuroR

   Plasmid

   #83889

   Purpose

   Lentiviral Sp dCas9-p300-P2A-PuroR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
   pac from Streptomyces alboniger
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EFS

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHL-EF1a-SphcCas9(D10A)-iP-A

   Plasmid

   #60600

   Purpose

   Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Inserts

   CRISPR Cas9 D10A
   puromycin resistance gene
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Changed Asp 10 to Ala, Codon usage optimized for …

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSc1-puro

   Plasmid

   #80438

   Purpose

   Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin

   Depositor

   Ervin Welker

   Article

   Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

   Inserts

   sp Cas9 gRNA
   eGFP
   Puromycin resistance
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV, U6, and hPGK

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSuiAb

   Plasmid

   #226171

   Purpose

   multiple resistance marker with apramycin and kanamycin

   Depositor

   Woojun Park

   Insert

   apramycin resistance gene
   

   Expression

   Bacterial

   Promoter

   pBR322ori

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTACT13

   Plasmid

   #24784

   Depositor

   Tanya Parish

   Insert

   PAg85a-TetR (tetR Bacterial)
   

   Use

   Mycobacterial shuttle vector

   Available Since

   Aug. 6, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVI

   Plasmid

   #31597

   Depositor

   Michele Calos

   Insert

   phiC31 integrase (int Streptomyces phage)
   

   Expression

   Mammalian

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAIP4_4O6G

   Plasmid

   #234175

   Purpose

   Heterologous protein expression of uncharacterized protein in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   4O6G
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pWS175

   Plasmid

   #90962

   Purpose

   Cas9 gap repair vector - HygR

   Depositor

   Tom Ellis

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRosettav2

   Plasmid

   #115557

   Purpose

   To determine correct antibiotic selection conditions in mammalian cells; standard for titering experiments

   Depositor

   John Doench

   Insert

   Hygromycin-Blasticidin-Puromycin-EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLAM12

    Plasmid

    #26908

    Depositor

    Graham Hatfull

    Article

    van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

    Insert

    Acetamidase Promoter
    

    Expression

    Bacterial

    Available Since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pWS173

    Plasmid

    #90960

    Purpose

    Cas9 gap repair vector - KanR

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-TET-MKOS

    Plasmid

    #20959

    Purpose

    piggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for creation of induced pluripotent stem cells

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    cMyc, KLF4, Oct4, Sox2 (Myc, Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    piggybac

    Tags

    IRES-bGeo

    Expression

    Mammalian

    Available Since

    April 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAIP4_1LU4

    Plasmid

    #234207

    Purpose

    Heterologous protein expression of soluble secreted antigen MPT53 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1LU4 (mpt53 Mycobacterium tuberculosis)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGR475

    Plasmid

    #213784

    Purpose

    Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker

    Depositor

    Chang Liu

    Insert

    WT TP-DNAP1 (recoded)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG25

    Plasmid

    #35121

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    natMX4
    

    Use

    Yeast replicating

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG35

    Plasmid

    #35123

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    natMX3
    

    Use

    Yeast replicating

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG36

    Plasmid

    #35126

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    nat1
    

    Use

    Yeast replicating

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQ2aB

    Plasmid

    #124887

    Purpose

    Basic Retroviral backbone with multiple 2a sites, linkers, BFP and and IRES_puromycin from pQCXIP

    Depositor

    Nevil Singh

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Blue Flourescent Protein, Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG31

    Plasmid

    #35124

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    patMX3
    

    Use

    Yeast replicating

    Available Since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMEND-Lx

    Plasmid

    #25010

    Depositor

    Brian Robertson

    Article

    Williams et al Plasmid. 2010 Sep;64(2):69-73. doi: 10.1

    Insert

    pMEND-Lx
    

    Expression

    Bacterial

    Available Since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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