We narrowed to 14,039 results for: TIM

1. pCRISPR-cBEST-v2-kasOP*

   Plasmid

   #209447

   Purpose

   improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

   Depositor

   Tilmann Weber

   Article

   Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

   Insert

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   PtipA; sgRNA expression from kasOP* promoter

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

   Plasmid

   #189633

   Purpose

   Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

   Plasmid

   #189632

   Purpose

   Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a(+)-6xHis-TEV-ORF9c

   Plasmid

   #201023

   Purpose

   Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   open reading frame 9c
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNIC28-HiBIT-WDR5

   Plasmid

   #200883

   Purpose

   Bacterial expression of N-terminal HiBIT-tagged human WDR5 protein

   Depositor

   Stefan Knapp

   Article

   Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

   Insert

   HiBIT-WDR5 (WDR5 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pShuttle.CC9

   Plasmid

   #192931

   Purpose

   Gateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expression

   Depositor

   Thorsten Stiewe

   Article

   Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

   Type

   Empty backbone

   Use

   Gateway-compatible cloning vector

   Promoter

   CMV

   Available Since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNB_EA_R1CiM_4EGFP_R3EGFPi2

   Plasmid

   #185968

   Purpose

   Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

   Depositor

   Sangram Bagh

   Article

   Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

   Insert

   Molecular IMPLY gate
   

   Use

   Synthetic Biology

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2xNLS-NLP-cMyc AspCas12a

   Plasmid

   #182120

   Purpose

   pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   2xNLS-NLP-cMyc AspCas12a
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-ATG13 (1-197)

   Plasmid

   #188074

   Purpose

   To make ATG101/ATG13 HORMA complex for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Expression

   Mammalian

   Mutation

   codon-optimized ATG13 (1-197)

   Promoter

   CMV

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX458-MMETn-del-gRNA2

    Plasmid

    #185007

    Purpose

    expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    MMETn-del-gRNA2
    

    Use

    CRISPR

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458-IAP-del-gRNA1

    Plasmid

    #185002

    Purpose

    expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    IAP-del-gRNA1 (Akp3 Mouse)
    

    Use

    CRISPR

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX458-MMERVK9C-del-gRNA1

    Plasmid

    #185004

    Purpose

    expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    MMERVK9C-del-gRNA1
    

    Use

    CRISPR

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX458-MMETn-del-gRNA1

    Plasmid

    #185006

    Purpose

    expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    MMETn-del-gRNA1
    

    Use

    CRISPR

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX458-MMERVK9C-del-gRNA2

    Plasmid

    #185005

    Purpose

    expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    MMERVK9C-del-gRNA2
    

    Use

    CRISPR

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX458-IAP-del-gRNA2

    Plasmid

    #185003

    Purpose

    expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    IAP-del-gRNA2 (Akp3 Mouse)
    

    Use

    CRISPR

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. TOMM20-mTurquoise-RhoA-G14V-deltaCaaX

    Plasmid

    #176123

    Purpose

    Constitutively active, mitochondrial localized RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    RHOA (RHOA Human)
    

    Expression

    Mammalian

    Mutation

    G14V

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS313_ATP3G919C

    Plasmid

    #120255

    Purpose

    Expression of ATP3-7 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    G919 changed to C; Alanine 307 changed to Proline

    Promoter

    ATP3

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS313_ATP3mut

    Plasmid

    #120256

    Purpose

    Expression of ATP3-67 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Isoleucine 304 changed to Asparagine; Alanine 307…

    Promoter

    ATP3

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBI-CMV1-mir144~451-CMV2-mir1

    Plasmid

    #164664

    Purpose

    Express miR-144~451 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144~451 (MIR451A, MIR144 Human)
    miR-1-1 (MIR1-1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA™5/FRT/CMV_promoter-ciCas9-E2A-mRFP

    Plasmid

    #164132

    Purpose

    Expresses ciCas9 and mRFP in mammalian cells (an insertion vector to generate ciCas9-knockin cell lines)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    ciCas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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