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pcDNA5-FRT-TO-FH-Nsp16

Plasmid

#157724

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp16 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp12

Plasmid

#157710

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp12 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp5-HF

Plasmid

#157687

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp5-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp5

Plasmid

#157686

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp5 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLJM1-PCYT2

Plasmid

#81074

Purpose

Expresses PCYT2 in mammalian cells

Depositor

Erin O'Shea

Publication

Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

Insert

phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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myr-FLAG-PIK3CA-pcw107

Plasmid

#64605

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

PIK3CA (PIK3CA Bovine)

Use

Lentiviral

Tags

FLAG and myristoylated

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Nano-lantern(cAMP-1.6)/pRSETB

Plasmid

#53591

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Publication

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Tags

6xHis

Expression

Bacterial

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pET28a-UVRAG CC

Plasmid

#24400

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

UVRAG (UVRAG Human)

Tags

His

Expression

Bacterial

Mutation

Contains on the CC domain, amino acids 200-270

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pFastBac-His-Tipin

Plasmid

#22891

Depositor

Aziz Sancar

Publication

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Tags

His

Expression

Insect

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
peGFP-N2-rPrmp

Plasmid

#22735

Depositor

Lynn Hudson

Publication

Nielsen et al J Neurosci. 2006 Sep 27. 26(39):9881-91.

Insert

Proline-rich transmembrane protein

Tags

EGFP

Expression

Mammalian

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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human K18

Plasmid

#14741

Depositor

M Bishr Omary

Publication

Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Keratin 18 (KRT18 Human)

Expression

Mammalian

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pPB_TRE3G_scFV-sfGFP-Kmt5cFL

Plasmid

#235581

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Kmt5c fused with scFV to programme H4K20me3

Depositor

Jamie Hackett

Publication

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Kmt5c (Kmt5c Mouse)

Use

CRISPR

Available since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pACS145

Plasmid

#204600

Purpose

Histone H3.3K36M-V5

Depositor

Tom Misteli

Publication

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.3 K36M (H3-3A Human)

Use

Lentiviral

Tags

human histone H3.3 K36M - V5

Expression

Mammalian

Mutation

histone H3.3 K36M

Promoter

CMV

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLVNP3.0-PEmaxRH

Plasmid

#206884

Purpose

Expresses FLAG-tagged PEmax (lacking the RNAseH domain) fused to Gag through a linker sequence

Depositor

Jacob Giehm Mikkelsen

Publication

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PEMax Delta RNAseH

Tags

FLAG

Promoter

CMV

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGBW-m4134547

Plasmid

#152583

Purpose

Mammalian expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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Myo 19-GFP

Plasmid

#134988

Purpose

Expression of human Myo 19 in mammalian cells.

Depositor

Martin Bähler

Publication

Oeding et al J Cell Sci. 2018 Sep 10;131(17). pii: jc

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Myo 19 (MYO19 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA6-N-3XFLAG-Axin1

Plasmid

#123463

Depositor

Bruce Beutler

Publication

Choi et al Science. 2019 May 10;364(6440). pii: 364

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Axin 1 (Axin1 Mouse)

Tags

FLAG

Expression

Mammalian

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

Plasmid

#113681

Purpose

A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Publication

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST- IP2.0(codon for C.elegans)

Plasmid

#109216

Purpose

IP2.0 for C. elegans (Codon optimization and introns).

Depositor

Takeshi Ishihara

Publication

Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

Insert

IP2.0 (Inverse Pericam 2.0)

Tags

His-tag

Expression

Worm

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pMEL10

Plasmid

#107916

Purpose

URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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