pDEST- IP2.0(codon for C.elegans)
(Plasmid
#109216)
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PurposeIP2.0 for C. elegans (Codon optimization and introns).
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109216 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneCustomized Vector
- Total vector size (bp) 6515
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIP2.0 (Inverse Pericam 2.0)
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SpeciesSynthetic
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Insert Size (bp)1560
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Tag
/ Fusion Protein
- His-tag (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note the IP2.0 nucleotide sequence in this plasmid contains 3 introns, which are removed prior to translation of the IP2.0 protein. For more information on the location of the introns, please see the annotated GenBank file.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST- IP2.0(codon for C.elegans) was a gift from Takeshi Ishihara (Addgene plasmid # 109216 ; http://n2t.net/addgene:109216 ; RRID:Addgene_109216) -
For your References section:
An improved inverse-type Ca2+ indicator can detect putative neuronal inhibition in Caenorhabditis elegans by increasing signal intensity upon Ca2+ decrease. Hara-Kuge S, Nishihara T, Matsuda T, Kitazono T, Teramoto T, Nagai T, Ishihara T. PLoS One. 2018 Apr 25;13(4):e0194707. doi: 10.1371/journal.pone.0194707. eCollection 2018. 10.1371/journal.pone.0194707 PubMed 29694380
Map uploaded by the depositor.
