We narrowed to 14,039 results for: TIM

1. pKM33

   Plasmid

   #154167

   Purpose

   Aedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Rel2 (long isoform)
   

   Tags

   FLAG

   Expression

   Insect

   Mutation

   C-terminal IκB domain deleted

   Promoter

   IE1 promoter and hr5 enhancer from Autographa cal…

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. INCTbiosyn-pUB-HspINT4

   Plasmid

   #127513

   Purpose

   Plasmid encodes H. sapiens codon optimized Integrase 4.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase 4 coding sequence codon optimized for H. sapiens expression.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. INCTbiosyn-pUB-HspINT5

   Plasmid

   #127514

   Purpose

   Plasmid encodes H. sapiens codon optimized Integrase 5.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase 5 coding sequence codon optimized for H. sapiens expression.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)

   Plasmid

   #133796

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

   Insert

   human codon optimized AcrIIA1 (truncated C-terminal domain only)
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   C-terminal domain only

   Promoter

   CMV and T7

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)

   Plasmid

   #133798

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

   Insert

   human codon optimized AcrIIA1(T114A/F115A)
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   T114A/F115A

   Promoter

   CMV and T7

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5FRTTO-NHASt-codoptUL33

   Plasmid

   #135103

   Purpose

   Codon-optimized HSV-1 UL33 with N-terminal HAStrep tag

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Insert

   HSV-1 UL33 codon optimized
   

   Tags

   HA-Strep-Strep

   Expression

   Mammalian

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5FRTTO-codoptUL50-CStHA

   Plasmid

   #135105

   Purpose

   Codon-optimized HSV-1 UL50 with C-terminal StrepHA tag

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Insert

   HSV-1 UL50 codon optimized
   

   Tags

   Strep-Strep-HA

   Expression

   Mammalian

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5FRTTO-codoptUL33-CStHA

   Plasmid

   #135106

   Purpose

   Codon-optimized HSV-1 UL33 with C-terminal StrepHA tag

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Insert

   HSV-1 UL33 codon optimized
   

   Tags

   Strep-Strep-HA

   Expression

   Mammalian

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5FRTTO-codoptUS2-CStHA

   Plasmid

   #135107

   Purpose

   Codon-optimized HSV-1 US2 with C-terminal StrepHA tag

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Insert

   HSV-1 US2 codon optimized
   

   Tags

   Strep-Strep-HA

   Expression

   Mammalian

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAWp63-clone3-12 (OptiHF-SpCas9)

    Plasmid

    #131737

    Purpose

    Expresses OptiHF-SpCas9 in mammalian cells

    Depositor

    Alan Siu Lun Wong

    Article

    Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

    Insert

    OptiHF-SpCas9
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    Q695A+K848A+E923M+T924V+Q926A

    Promoter

    EFS

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. GS2.1/EC

    Plasmid

    #132568

    Purpose

    sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    egg cell promoter, Cas9-mApple, pds3 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    A. thaliana egg cell promoter

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Met133I, M1381I, M186I ELL2

    Plasmid

    #127263

    Purpose

    For in vitro translation of human ELL2 with Met133I, M1381I, M186I

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Three Mets (133, 138, 186) to Ileu

    Promoter

    T7

    Available Since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 563aa ELL2 pEF4B

    Plasmid

    #127273

    Purpose

    Expresses 563aa human WT ELL2

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 truncated at SphI (ELL2 Human)
    

    Expression

    Mammalian

    Mutation

    Contains 564 N-terminal amino acids ELL2, termina…

    Promoter

    pEF1a

    Available Since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTAMAHISTEV_Ab-PirA

    Plasmid

    #128959

    Purpose

    expresses Acinetobacter baumannii PirA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    ferric enterobactin receptor PirA
    

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Designed HEL-binding scFv D44.1des

    Plasmid

    #111719

    Purpose

    Yeast Surface Display of D44.1des

    Depositor

    Sarel Fleishman

    Article

    Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

    Insert

    D44.1 des
    

    Tags

    cmyc tag

    Expression

    Yeast

    Mutation

    L Q89G, L S55V, L S43P, H V37M, H H43G, H W47Y, H…

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA tagged Sph ELL2

    Plasmid

    #127270

    Purpose

    For in vitro translation of human WT ELL2 with internal HA tag

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    WT Mets and HA tag at SphI site (8th Met)

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA tagged mid ELL2

    Plasmid

    #127274

    Purpose

    Expresses human ELL2 with HA tag in the middle that does not disrupt function

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 with HA at XbaI (ELL2 Human)
    

    Expression

    Mammalian

    Mutation

    HA inserted at XbaI after Met 186

    Promoter

    pEF1a

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Met138ILeu ELL2

    Plasmid

    #127260

    Purpose

    For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met138 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Met138 to Ileu Quikchange

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Met186ILeu ELL2

    Plasmid

    #127261

    Purpose

    For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met186 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Met186 to Ileu Quikchange

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Met138ILeu, Met186ILeu ELL2

    Plasmid

    #127265

    Purpose

    For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Two Mets (138,186) to ILeu Quikchange

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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