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pEM-eCas9-D1135E

Plasmid

#154926

Purpose

aTc inducible eCas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

eCas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

K848A, K1003A, R1060A, D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-OptiCas9-D1135E

Plasmid

#154928

Purpose

aTc inducible OptiCas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

OptiCas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

R661A, K1003H, D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9-D1135E

Plasmid

#154931

Purpose

aTc inducible Cas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

Cas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-pheT

Plasmid

#154920

Purpose

aTc inducible gRNA targeting pheT along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting pheT

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-mqo

Plasmid

#154921

Purpose

aTc inducible gRNA targeting mqo along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting mqo

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-hisF

Plasmid

#154922

Purpose

aTc inducible gRNA targeting hisF along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting hisF

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-ampG

Plasmid

#154923

Purpose

aTc inducible gRNA targeting ampG along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting ampG

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-mCherry

Plasmid

#127058

Purpose

Low phosphate inducible mCherry with N-terminal linker and His tag

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

mCherry

Expression

Bacterial

Promoter

yibDp

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-GFPuv

Plasmid

#127078

Purpose

Low phosphate inducible yibD gene promoter expressing GFPuv reporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPuv

Expression

Bacterial

Promoter

yibDp

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-ald*-alaE

Plasmid

#134939

Purpose

Low phosphate inducible alanine dehydrogenase and alanine exporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

alanine dehydrogenase and L-alanine exporter

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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