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pRK5-mEGFP-HMGB1-MUT-IDR2

Plasmid

#194552

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-Patchless

Plasmid

#194553

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "Patchless" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44, truncation by stop codon after Lys209

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau E14 P301L

Plasmid

#46909

Purpose

expresses EGFP tagged Tau E14 P301L in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau E14 P301L (MAPT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

P301L AND all 14 S/P or T/P amino acid residues (…

Promoter

CMV

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5 BMPS HK318/9EE-6xHis

Plasmid

#204836

Purpose

Mammalian expression of recombinant BMPS WT

Depositor

Monther Abu-Remaileh

Article

Medoh et al Science. 2023 Sep 15;381(6663):1182-1189

Insert

CLN5 (CLN5 Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

HK318/9EE

Promoter

CMV

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag Leucyl tRNA synthetase pRK5

Plasmid

#46341

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

leucyl tRNA synthetase (LARS1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pRK5 FLAG DEPTOR (PDZ domain)

Plasmid

#21701

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 13. ():.

Insert

DEPTOR PDZ domain (DEPTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

PDZ domain only

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG DEPTOR (DEP domains)

Plasmid

#21700

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 13. ():.

Insert

DEPTOR DEP domains (DEPTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

DEP domains only

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro1 Δ593-618

Plasmid

#47895

Purpose

Expresses myc tagged Miro1 lacking transmembrane domain

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro1 Δ593-618 (RHOT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Δ593-618, lacks transmembrane domain

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-mEGFP-HMGB1-MUT-IDR

Plasmid

#194551

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
prk5-MFSD12-TEV-3xFLAG-3xHA

Plasmid

#149504

Purpose

Transient expression of human MFSD12 with recoded nucleotide sequence.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

MFSD12 (MFSD12 Human)

Use

Transient expression

Tags

TEV-3XHA-3XFLAG

Expression

Mammalian

Mutation

Codon Optimized Sequence

Promoter

CMV

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-RtoK

Plasmid

#194559

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R > K" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

R > K substitutions after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mEGFP-HMGB1-MUT-KtoR

Plasmid

#194560

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "K > R" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K > R substitutions after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-myc-Miro2 Δ593-618

Plasmid

#47901

Purpose

Expresses myc tagged Miro2 lacking transmembrane domain

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 Δ593-618 (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Δ593-618, lacks transmembrane domain. Also see De…

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
HA GST F5A deltaCT S6K1 pRK5

Plasmid

#8467

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

f5a delta CT p85 s6k1 (Rps6kb1 Rat)

Use

Tags

ha/gst

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-SZT2 2241-end

Plasmid

#87039

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AA 2241-end

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-GST-Presc-mAkt1 (S473T)

Plasmid

#48806

Purpose

expresses mutant Akt1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

Akt1 (Akt1 Mouse)

Use

Tags

HA-GST-Presc

Expression

Mammalian

Mutation

S473T

Promoter

CMV

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pRK5 Myc-tagged PSK (K57A)

Plasmid

#197150

Purpose

Expression of kinase-deficient MYC-tagged PSK1-alpha (K57A) / TAOK2 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

Insert

TAOK2 (TAOK2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

SV40

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-PSK2 (1-416) (K57A)

Plasmid

#197124

Purpose

Expression of kinase-deficient Myc-tagged PSK2 (aa1-416, K57A) / TAOK1 (aa1-416, K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Tavares et al J Biol Chem. 2013 May 24;288(21):15418-2

Insert

TAOK1 (aa1-416, K57A) (TAOK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Changed K 57 to A and deleted C-terminus

Promoter

SV40

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-Rdel

Plasmid

#194556

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R del" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Deletion of Arginines after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mEGFP-HMGB1-MUT-RorKtoA

Plasmid

#194558

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R or K > A" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

R or K > A substitutions after Lys185

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge