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Plasmid#103459PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-340-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-340-5p target (MIR340 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-135b-5p
Plasmid#103229PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-135b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-135b-5p target (MIR135B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-160
Plasmid#74607PurposeExpresses Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
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LSB-hsa-miR-30e-5p
Plasmid#103419PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30e-5p target (MIR30E Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30a-5p
Plasmid#103410PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30a-5p target (MIR30A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-615-5p
Plasmid#103675PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-615-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-615-5p target (MIR615 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-Patronin CC 638–1457
Plasmid#59051Purposefor baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)DepositorInsertPatronin (Patronin Fly)
UseTagseGFPExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-Patronin CC 776–1457
Plasmid#59052Purposefor baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)DepositorInsertPatronin (Patronin Fly)
UseTagseGFPExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-584-5p
Plasmid#103665PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-584-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-584-5p target (MIR584 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7g-5p
Plasmid#103158PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7g-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7g-5p target (MIRLET7G Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
ATF4 9: (SL+)5’ATF4.GFP
Plasmid#21856DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationStem loop introduced at the 5' end of the mR…PromoterAvailable sinceSept. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-125a-5p
Plasmid#103188PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-125a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-125a-5p target (MIR125A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW322
Plasmid#115953PurposeDestination VectorDepositorInsertLvl1 P1 With 3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW323
Plasmid#115954PurposeDestination VectorDepositorInsertLvl1 P1 No 3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW325
Plasmid#115956PurposeDestination VectorDepositorInsertLvl1 P2 With 3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW326
Plasmid#115957PurposeDestination VectorDepositorInsertLvl1 P2 No 3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW327
Plasmid#115958PurposeDestination VectorDepositorInsertLvl1 P2 End
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW328
Plasmid#115959PurposeDestination VectorDepositorInsertLvl1 P3 With E1-3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW088
Plasmid#115936Purpose3'UTR insert for replicon MoCloDepositorInsertLvl0 3'UTR No E1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only