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Plasmid Kit#1000000171PurposeVectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.DepositorAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only
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Toronto KnockOut Library (TKOv1)
Pooled Library#1000000069PurposeCRISPR gRNA knockout pooled library for targeting the human genomeDepositorAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Toronto KnockOut version 3 library (TKOv3)
Pooled Library#90294PurposeOptimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1DepositorAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 RBD Omicron BA.1 SSM Library
Pooled Library#1000000187DepositorAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 RBD Omicron BA.2 SSM Library
Pooled Library#1000000188DepositorAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse Toronto KnockOut (mTKO) CRISPR Library - lentiCRISPRv2
Pooled Library#159392PurposeMouse lentiviral genome-wide CRISPR libraryDepositorExpressionMammalianSpeciesMus musculusUseCRISPR and LentiviralAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mouse Toronto KnockOut (mTKO) CRISPR Library - pLCKO2
Pooled Library#159393PurposeMouse lentiviral genome-wide CRISPR libraryDepositorExpressionMammalianSpeciesMus musculusUseCRISPR and LentiviralAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human Genome-wide Intron Tagging Library, Frame 1
Pooled Library#216134PurposeLibrary of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human Genome-wide Intron Tagging Library, Frame 0
Pooled Library#216133PurposeLibrary of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRL-SV40P
Plasmid#27163PurposeRenilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experimentsDepositorInsertSV40 promoter
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-24xGCN4_v4-sfGFP
Plasmid#61058PurposeA direct fusion of sfGFP to the 24xGCN4_v4 peptide arrayDepositorInsert24xGCN4_v4 peptide array and sfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV BiP-Myc-KDEL-wt
Plasmid#27164PurposeMammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequenceDepositorInsertBiP (HSPA5 Human)
UseTagsKDEL and MycExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
p5xATF6-GL3
Plasmid#11976PurposeReporter gene with five ATF6 sites that is highly sensitive to ER stressDepositorInsert5xATF6 binding site
UseTagsLuc and c-fos promoterExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
3XMEF2-luc
Plasmid#32967PurposeReporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoterDepositorInsertMEF2 binding sites (MEF2A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterMEF2Available sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
ERroGFP_iE_pCDNA3
Plasmid#47954PurposeMammalian expression vector encoding for the ER targeted and FLAGM1 tagged redox probe roGFPiE (roGFP C147, E147b, C204)DepositorInsertERroGFP
UseTagsFLAGExpressionMammalianMutationinsertion E147BPromoterAvailable sinceOct. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7a-5p
Plasmid#103146PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7a-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7d-5p
Plasmid#103151PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7d-5p target (MIRLET7D Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-dWPRE
Plasmid#60907PurposeThe plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imagingDepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
Plasmid#60904PurposeThe plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64DepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -