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Showing: 461 - 480 of 1655 results

  1. AAV-EF1α1.1-FLPX-rc [Chronos-tdTomato]

    Plasmid
    #122103
    Purpose
    AAV-mediated expression of Chronos-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    Chronos-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a（1.1kb short version)
    Available since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    122103-plasmid-map-sequence-id-256678

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  2. pAAV-EF1α1.1-FLEX-rc [ChrimsonR-GFP]

    Plasmid
    #108273
    Purpose
    AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-GFP
    Use
    AAV
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1α promoter (1.1kb short version)
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    108273-plasmid-map-sequence-id-213551

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  3. pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]

    Plasmid
    #84485
    Purpose
    AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    Chronos-GFP
    Use
    AAV
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α, 1.1 kb long (short version)
    Available since
    April 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    84485-plasmid-map-sequence-id-176951

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  6. pEP4 E02S EN2K

    Plasmid
    #20925
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4
    Depositor
    James Thomson
    Article
    Yu et al Science. 2009 Mar 26.
    Insert
    Oct4 and Sox2; Nanog and Klf4 (SOX2, POU5F1, NANOG, KLF4 Human)
    Use
    Episomal/ebna
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    20925-plasmid-map-sequence-id-9761

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  7. pAAV-EF1α1.1-ChrimsonR-tdTomato

    Plasmid
    #122064
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α promoter (1.1kb short version).tdTomato has codons varied to reduce recombination. Using SV40 pA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1a（1.1kb short version)
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122064-plasmid-map-sequence-id-242270

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  9. pN1 Lck-GCaMP5G

    Plasmid
    #34924
    Purpose
    Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane
    Depositor
    Baljit Khakh
    Article
    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.
    Insert
    Lck-GCaMP5G
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Plasmid contains N-terminal 26 amino acid membran…
    Promoter
    CMV
    Available since
    Feb. 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    34924-plasmid-map-sequence-id-42429

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  12. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid
    #62723
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Has Service
    AAV1 and AAV5
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    human synapsin promoter
    Available since
    April 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62723-plasmid-map-sequence-id-166279

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  14. pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]

    Plasmid
    #84483
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    human synapsin promoter
    Available since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84483-plasmid-map-sequence-id-177044

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  15. AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid
    #128587
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1α (1.1 kb short version)
    Available since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    128587-plasmid-map-sequence-id-257676

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  17. pEP4 E02S EM2K

    Plasmid
    #20923
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4
    Depositor
    James Thomson
    Article
    Yu et al Science. 2009 Mar 26.
    Insert
    Oct4 and Sox2; Myc and Klf4 (SOX2, KLF4, MYC, POU5F1 Human)
    Use
    Episomal/ebna
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    20923-plasmid-map-sequence-id-272720

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Showing: 461 - 480 of 1655 results