We narrowed to 2,501 results for: mia

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pLAS2w.Phyg-CHST8

Plasmid

#232650

Purpose

expresses CHST8 wild-type in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CHST8 (CHST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLAS2w.Phyg-CHST8 (3A)

Plasmid

#232651

Purpose

expresses CHST8 REP-to-AAA mutant in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CHST8 (3A) (CHST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R258A, E259A, P260A

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pPB9P2

Plasmid

#210030

Purpose

Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

prime editor 2
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2D-A

Plasmid

#221713

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D683A in active site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2F-Y

Plasmid

#221722

Purpose

Expression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2F-Y (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

F666Y in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2E-D

Plasmid

#221721

Purpose

Expression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2E-D (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

E637D in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2D-A

Plasmid

#221720

Purpose

Expression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2D-A (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

D669A in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2Y-F

Plasmid

#221715

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

Y680F in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2D-E

Plasmid

#221714

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D651E in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pSHDY*in_PpetE:BCD2:AgBIS:Flag

Plasmid

#133971

Purpose

Heterologous, copper-inducible expression of (E)-α-bisabolene synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. The AgBIS CDS is fused to a C-termial Flag-tag.

Depositor

Pia Lindberg

Article

Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

Insert

AgBIS:Flag

Use

Synthetic Biology

Tags

Flag

Expression

Bacterial

Mutation

inserted gene is codon optimized for Synechocyst…

Promoter

PpetE:BCD2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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