We narrowed to 13,725 results for: cas9

1. pU6-Universal

   Plasmid

   #52694

   Purpose

   This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

   Depositor

   Sebastian Lourido

   Inserts

   Cas9
   Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
   

   Use

   CRISPR; Toxoplasma gondii

   Tags

   3X FLAG and Nuclear localization signal

   Expression

   Mutation

   Promoter

   TgTUB1 and Toxoplasma U6

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJAT77

   Plasmid

   #204295

   Purpose

   pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRB1017

   Plasmid

   #59936

   Purpose

   empty vector for gRNA cloning

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. LRG2.1-CyOFP1

   Plasmid

   #124771

   Purpose

   3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUGW NLS-Cre

   Plasmid

   #183425

   Purpose

   Lentiviral Cre vector

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   NLS-Cre
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYTK-DN6

   Plasmid

   #180287

   Purpose

   HygR multi-gene yeast expression vector (GFP-dropout)

   Depositor

   Andreas Milias-Argeitis

   Article

   Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

   Insert

   ConLS' - GFP - ConRE' - HygR - CEN6/ARS4
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. gRNA-eGFP-Reporter

   Plasmid

   #60719

   Purpose

   Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and mouse U6

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH372

   Plasmid

   #86839

   Purpose

   mamalian expression of AcrIIA1

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMiniT:GFP-Gephyrin TKIT donor

   Plasmid

   #169433

   Purpose

   Donor DNA for TKIT knockin of GFP to Gephyrin in mouse

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYTK-DN5

    Plasmid

    #180286

    Purpose

    NatR multi-gene yeast expression vector (GFP-dropout)

    Depositor

    Andreas Milias-Argeitis

    Article

    Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

    Insert

    ConLS' - GFP - ConRE' - NatR - CEN6/ARS4
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB8644

    Plasmid

    #126919

    Purpose

    Plasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYB

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI and HMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LRG2.1-mOrange

    Plasmid

    #124772

    Purpose

    3rd generation lentiviral plasmid to co-express a guide RNA and mOrange

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB8787

    Plasmid

    #126921

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    HMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. vc241

    Plasmid

    #193453

    Purpose

    Expression of rRFP in A. castellanii

    Depositor

    Chantal Abergel

    Article

    Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

    Insert

    mRFP
    

    Use

    Acanthamoeba exression

    Tags

    Expression

    Mutation

    Promoter

    GAPDH

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB8748

    Plasmid

    #126918

    Purpose

    Plasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtE

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtE
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDD363

    Plasmid

    #91829

    Purpose

    LoxP-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    LoxP-SEC-LoxP
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDD379

    Plasmid

    #91834

    Purpose

    SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCfB8788

    Plasmid

    #126922

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI and HMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSEVA1213S

    Plasmid

    #122095

    Purpose

    constitutive expression of I-SceI for genome engineering in Pseudomonas

    Depositor

    Pablo Ivan Nikel

    Article

    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    I-SceI restriction endonuclease
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7sgRNA

    Plasmid

    #111820

    Purpose

    Expresses sgRNA in T. brucei cells

    Depositor

    David Horn

    Article

    Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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