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Ensconsin-18-mEos2

Plasmid

#99230

Purpose

Mammalian expression of the photoconvertible protein mEos2 as an Ensconsin fusion.

Depositor

Periklis Pantazis

Publication

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

mEos2

Tags

Ensconsin (MAP7)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFPE9

Plasmid

#83545

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter-yEGFP

Expression

Yeast

Promoter

TEF1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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3XFlag-pLPC

Plasmid

#73560

Purpose

vector encoding 3 Flag tags

Depositor

Gerardo Ferbeyre

Publication

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

3X-Flag

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAAV-Ef1a-DIO_EGFP-RandE3

Plasmid

#79872

Purpose

For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

Depositor

Don Arnold

Publication

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-RandE3

Use

AAV, Cre/Lox, and Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Promoter

EF1a

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIKneo 3XFLAG-wtGATA6-3XAU1_RNAi resistant

Plasmid

#72616

Purpose

Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged wtGATA6

Depositor

Kevin Janes

Publication

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA Binding Protein 6 (GATA6 Human)

Use

Lentiviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Promoter

TRE tight

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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p413GAL1-Zipp.-L-IFP-F[2] (Yeast)

Plasmid

#52893

Purpose

Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Publication

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Zipp.-L-IFP-F[2]

Tags

L-IFP-F[2]

Expression

Yeast

Promoter

GAL1

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-NLS-dCas13-NLS-eNAT10

Plasmid

#238300

Purpose

Expresses nuclear version of the programmable RNA acetylation system.

Depositor

Won Do Heo

Publication

Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

Insert

dPspCas13b fused to eNAT10 (NAT10 Human, Prevotella sp. P5-125)

Use

CRISPR

Tags

SV40 BPNLS and SV40 BPNLS (C terminal of dPspCas1…

Expression

Mammalian

Mutation

H133A for catalytically inactive mutant, deletion…

Available since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSM41

Plasmid

#235728

Purpose

Protein expression of FL His6- human VPS34 in bacteria

Depositor

Roger Williams

Publication

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Insert

VPS34 (PIK3C3 Human)

Tags

6xHis-TEV

Expression

Bacterial

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmEGFP-N1

Plasmid

#217486

Purpose

Expression of monomeric Enhanced Green Fluorescent Protein

Depositor

Bertrand Collet

Publication

Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

Insert

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMpGWBs02

Plasmid

#222478

Purpose

Gateway vector for 35s promoter-driven expression in Marchantia polymorpha carrying a sulfadiazine selection marker.

Depositor

Philip Carella

Publication

Robinson et al J Exp Bot. 2024 Jun 2:erae256. doi: 10.1

Type

Empty backbone

Expression

Plant

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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LifeAct-MTED

Plasmid

#217498

Purpose

Expresses LifeAct-mScarlet-MTED for targeting fluorescent, MT depolymerizing peptide MTED to f-actin

Depositor

Erik Dent

Publication

Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

Insert

LifeAct-mScarlet-MTED

Expression

Mammalian

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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plVX-EF1a-FLAG-hMETTL3-IRES-ZsGreen

Plasmid

#207123

Purpose

Lentiviral vector to express human METTL3

Depositor

Silvia Monticelli

Publication

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

hMETTL3 (METTL3 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSCALPS-FLAG-HA-mmuMettl3

Plasmid

#207122

Purpose

Lentiviral plasmid to express mouse Mettl3

Depositor

Silvia Monticelli

Publication

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Mettl3 (Mettl3 Mouse)

Use

Lentiviral

Tags

FLAG-HA

Promoter

SSFV

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_0_mKate2_synCoTC

Plasmid

#199469

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mKate2

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSA_2_mKate2_synCoTC

Plasmid

#199471

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mKate2

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSA_2_mTagBFP2_synCoTC

Plasmid

#199474

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mTagBFP2

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

Plasmid

#199483

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mKate2

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA5/FRT/TO-[4xCox8]-ChemoG-NAD

Plasmid

#193823

Purpose

CMV overexpression of ChemoG-NAD in the mitochondria of mammalian cell lines

Depositor

Julien Hiblot

Publication

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

[4xCox8]-ChemoG-NAD

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoB-NAD

Plasmid

#193824

Purpose

CMV overexpression of ChemoB-NAD in mammalian cell lines

Depositor

Julien Hiblot

Publication

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB-NAD

Expression

Mammalian

Mutation

EBFP2:N39Y, A206K, T225R; HT7: L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-HseIF4AI_AC

Plasmid

#148438

Purpose

Mammalian Expression of HseIF4AI

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HseIF4AI (EIF4A1 Human)

Expression

Mammalian

Mutation

3 silent mutations relative to NM_001416.2

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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