We narrowed to 8,324 results for: Set;

1. pBHt2G-RFP

   Plasmid

   #107162

   Purpose

   Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

   Depositor

   Sophien Kamoun

   Type

   Empty backbone

   Use

   Fungal grobacterium transformation plasmid, teste…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-galK

   Plasmid

   #193727

   Purpose

   Contains expression cassette for E.coli galK for use as a counterselection marker in strains lacking galK

   Depositor

   Martin Buck

   Insert

   galK (galK E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23113

   Available since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUPRT::Floxed DHFR-TS*

   Plasmid

   #100606

   Purpose

   TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.

   Depositor

   David Sibley

   Insert

   TgUPRT::Floxed TgDHFR-TS*
   

   Use

   Toxoplasma expression

   Tags

   Expression

   Mutation

   Pyrimethamine resistance

   Promoter

   pTgDHFR-TS

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIVTR(T7A)-CRE-T2A-PuroR

   Plasmid

   #172298

   Purpose

   In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes

   Depositor

   Patrik Verstreken

   Insert

   CRE-T2A-PuroR
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter A-initiating

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAW8-aid-3HA-Turg1:kanMX6

   Plasmid

   #169355

   Purpose

   C-terminal aid-3HA degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Use

   Tags

   3XHA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAW8-aid-V5-eDSR-Turg1:kanMX6

   Plasmid

   #169365

   Purpose

   C-terminal aid-V5-eDSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Use

   Tags

   1XV5

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAW8-aid-5Myc-Turg1:kanMX6

   Plasmid

   #169356

   Purpose

   C-terminal aid-5Myc degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Use

   Tags

   5XMyc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. SP65U1-301a

   Plasmid

   #105821

   Purpose

   plasmid for the overexpression of miR-301a under the U1 expression cassette inserted in the pSP65 plasmid

   Depositor

   Cecilia Mannironi

   Insert

   MIRNA301 (Mir301 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U1

   Available since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDual_dsCas9_Puro

   Plasmid

   #214680

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two empty sgRNA cloning sites

   Depositor

   Kevin Janes

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHflu1

    Plasmid

    #203878

    Purpose

    pSU20 with addition of CEN6-ARSH4-HIS3 cassette for S. cerevisiae and RK2 origin of conjugal transfer

    Depositor

    Bogumil Karas

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. cTRE-GFP-miR30_shRen

    Plasmid

    #135666

    Purpose

    Introduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shRenilla into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    shRenilla
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIVTR(T7A)-CRE-T2A-BleoR

    Plasmid

    #172300

    Purpose

    In vitro transcription of CRE-T2A-BleoR synthetic construct for the excision of floxed cassettes

    Depositor

    Patrik Verstreken

    Insert

    CRE-T2A-BleoR
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter A-initiating

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAW8-aid-V5-spo5DSR-Turg1:kanMX6

    Plasmid

    #169361

    Purpose

    C-terminal aid-V5-spo5DSR degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    1XV5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pREP81xV5_ccdB

    Plasmid

    #79019

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selction

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Use

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pREP82xV5_ccdB

    Plasmid

    #79020

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Use

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWPI

    Plasmid

    #12254

    Purpose

    2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.

    Depositor

    Didier Trono

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Tags

    EMCV IRES-EGFP cassette

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

    Plasmid

    #174893

    Purpose

    amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Insert

    mCherry and EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB Neo* PB

    Plasmid

    #138413

    Purpose

    Minimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

    Depositor

    Mario Capecchi

    Insert

    Neo*
    

    Use

    Mouse Targeting; Piggybac

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. G-RCAS (A)

    Plasmid

    #15182

    Depositor

    Connie Cepko

    Article

    Harpavat et al BMC Dev Biol. 2006 . 6():2.

    Insert

    Gateway cassette
    

    Use

    RNAi and Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDTA-5

    Plasmid

    #128845

    Purpose

    Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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