We narrowed to 159,530 results for: addgene

1. BPK2660

   Plasmid

   #70709

   Purpose

   Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFCcGi

   Plasmid

   #59324

   Purpose

   Inducible expression of GFP and constitutive expression of mCherry

   Depositor

   Sophie Helaine, David Holden

   Article

   Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

   Inserts

   mCherry
   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pbad and rpsM

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. hIRE1a K599A

   Plasmid

   #20745

   Depositor

   Fumihiko Urano

   Article

   Lipson et al PLoS ONE. 2008 . 3(2):e1648.

   Insert

   IRE1 (ERN1 Human)
   

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   K599A, dominant negative kinase mutant

   Promoter

   Available since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

4. hSyn-IRES2-GFP

   Plasmid

   #234875

   Purpose

   Lentiviral plasmid with a multiple cloning site followed by IRES2-GFP.

   Depositor

   Andrew Arrant

   Article

   Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSYn

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAG-Blimp1

   Plasmid

   #73995

   Purpose

   Plasmid expressing mouse Blimp1

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   Blimp1 gene (Prdm1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. H2B-MiniR

   Plasmid

   #208328

   Purpose

   MiniR genetically encoded tag introduced after H2B; to be used with a MiniE probe peptide.

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   H2B-MiniR
   

   Use

   Tags

   MiniR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. tdTomato-LacI

   Plasmid

   #183921

   Purpose

   Lac repressor labeled with tdTomato; can be used to label lacO sites

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdTomato-LacI (lacI Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PNCA-GFP-dYY1

   Plasmid

   #67791

   Purpose

   Use together with pCMV-intron and VSV-G to package MMLV reporter virus

   Depositor

   Stephen Goff

   Article

   Schlesinger et al Cell Rep. 2013 Jul 11;4(1):50-8. doi: 10

   Insert

   MMLV-LTR-GFP
   

   Use

   Tags

   Expression

   Mutation

   Deletion of LTR U3 YY1 binding domain

   Promoter

   Available since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pLJM1 Flag Raptor Rheb15

   Plasmid

   #26634

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   raptor-Rheb15 (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRISPR_HLA_class_I_1

    Plasmid

    #164986

    Purpose

    Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA class I
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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