We narrowed to 7,609 results for: Pol
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Plasmid#196859PurposeExpression of Cyclin dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) fused to tandem (td)-mCherry.DepositorInserttd-mCherry-Cdk5rap2 (Cdk5rap2 Rat)
ExpressionMammalianPromoterChicken bactin (plus Chicken bactin intron)Available SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HshnRNPE2iso3_Z
Plasmid#148118PurposeMammalian Expression of HshnRNPE2iso3DepositorInsertHshnRNPE2iso3 (PCBP2 Human)
ExpressionMammalianMutationone non silent mutation L239 to S compapred to th…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
secCitrine
Plasmid#181964PurposeBasic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoterDepositorInsertsignal peptide sequence from basic chitinase
TagsCitrine YFPExpressionPlantPromoter1.5 kb MUM4 promoterAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S D808N
Plasmid#169947PurposePurification of catalytically-dead DPP9S from insect cellsDepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
ADAP1
Plasmid#163905PurposeFull length mouse ADAP1 for purification from insect cellsDepositorAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ197
Plasmid#162670PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
TagsHisExpressionBacterialMutationS131G; codon optimized for expression in E. coli …PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPTG-GG-sg2+sg3
Plasmid#127199PurposePlasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoterDepositorInserttRNA-gRNA
UseEmpty sgrna plasmidExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_UG3
Plasmid#133874PurposeExpresses UG3 in mammalian cells.DepositorAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.16
Plasmid#133536PurposesfGFP driven by a T7 promoter with the tetO sequence 19 bp downstream from the promoter start siteDepositorInsertsfGFP
TagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.8
Plasmid#133521PurposesfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start siteDepositorInsertsfGFP
TagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available SinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBac(3xP3-gTc’v; pUb:lox-mYFP-lox-H2BmCherry)
Plasmid#119064PurposeCre/lox flip-out construct for marking cell clones in Tribolium castaneumDepositorInsertspBacL
pUb regulatory sequence
loxN
mYFP
loxN
H2B-mcherry
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR
ExpressionInsectAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC194
Plasmid#118882Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
TagsSNAP tag, cleavable with TEV protease, HIS6, clea…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC231
Plasmid#118885Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease and mCitrine…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC254
Plasmid#118889Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease; mCherry, cl…ExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A2-9
Plasmid#112825PurposepSMF2 with all S to A mutations in only repeats 2-9.DepositorInsertRPB1 (RPO21 Budding Yeast)
ExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 2…PromoterNative promoterAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTHFR (MTHFRA-c042)
Plasmid#110271PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-PC-2-CTS
Plasmid#86068PurposeA bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.DepositorInsertPolycystin-2 (Pkd2 Human)
TagsGSTExpressionBacterialMutationcontains the region from 1-15PromotertacAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHT-PRD
Plasmid#87755PurposeBacterial expression vector for producing 6x histidine tagged dynamin 1a proline rich domain (residues 746-864)DepositorInsertSemi-synthetic gene coding for dynamin 1a proline rich domain (residues 746-864)
Tags6X histidine tagExpressionBacterialPromoterT7 RNA polymerase promoterAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(chimpanzee)
Plasmid#79032PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(chimpanzee)
TagsOneSTrEP-FLAGExpressionInsectPromoterpolyhedrinAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB425
Plasmid#68690PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTriple repeats of Citrine (3xCitrine)ExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only