We narrowed to 28,960 results for: Tat
-
Plasmid#224349PurposeExpresses human KDAC8 (HDAC8) D101R in E. coliDepositorInsertKDAC8 (HDAC8 Human)
TagsTEV-cleavable His6ExpressionBacterialMutationAspartate 101 mutated to argininePromoterT5Available SinceOct. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJExpress401-KDAC8 D101A
Plasmid#224347PurposeExpresses human KDAC8 (HDAC8) D101A in E. coliDepositorInsertKDAC8 (HDAC8 Human)
TagsTEV-cleavable His6ExpressionBacterialMutationAspartate 101 mutated to alaninePromoterT5Available SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pU6-hL1-5'_3.3kb plasmid
Plasmid#226006PurposePlasmid used to generate DNA-FISH probe targeting at human L1HSDepositorInsertHuman L1 element 3.3kb sequences at the 5' end
UseSynthetic BiologyAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Ctr1-gRNA-EGFP
Plasmid#226000PurposeNegative control for CRISPRi-KDDepositorInsertnegtive control sgRNA of no specific targets in human genome
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Ctr2-gRNA-EGFP
Plasmid#226001PurposeNegative control for CRISPRi-KDDepositorInsertnegtive control sgRNA of no specific targets in human genome
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NCL-gRNA1-EGFP
Plasmid#226004PurposeCRISPRi-KD of human NucleolinDepositorInsertsgRNA targeting human Nucleolin (NCL Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NCL-gRNA2-EGFP
Plasmid#226005PurposeCRISPRi-KD of human NucleolinDepositorInsertsgRNA targeting human Nucleolin (NCL Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-455-WT
Plasmid#215881PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-455-WT (MIR455 Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-376a1(3p)-I-type
Plasmid#215883PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-I-type (MIR376A1 Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-589(5p)-I-type
Plasmid#215884PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-589(5p)-I-type (MIR589 Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-589(3p)-I-type
Plasmid#215885PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-589(3p)-I-type (MIR589 Human)
ExpressionMammalianAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-PODXL S481A
Plasmid#195223PurposeMammalian expression vector containing GFP tagged Podocalyxin. PKC phosphorylation-deficient mutant.DepositorAvailable SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML RING
Plasmid#185631Purposeexpression of GST-tagged PML RING domain in bacterial cellsDepositorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKS722A
Plasmid#216545PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: S722A) of human PTK2DepositorInsertPTK2 (S722A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation S722AAvailable SinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKY861F
Plasmid#216546PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: Y861F) of human PTK2DepositorInsertPTK2 (Y861F) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation Y861FAvailable SinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKS732A
Plasmid#216541PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: S732A) of human PTK2DepositorInsertPTK2 (S732A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation S732AAvailable SinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKS910A
Plasmid#216542PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: S910A) of human PTK2DepositorInsertPTK2 (S910A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation S910AAvailable SinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-miRFP670
Plasmid#220600PurposeConstitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.DepositorInsertmiRFP670
UseAAVTagsArgiNLSExpressionMammalianPromoterEF1aAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)
Plasmid#220597PurposeConstitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.DepositorInsertmVenus-Q69M (ME)
UseAAVTagsArgiNLSExpressionMammalianPromoterEF1aAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd
Plasmid#216171PurposeExpress the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR107
Plasmid#185037Purpose400 nt l31 promoter region, l31 5'UTR AGA74-76GAG mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationAGA74-76GAG mutation of rpmE 5'UTR, Only fir…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-SPNS1-sg1
Plasmid#198566PurposeSPNS1-targeting sgRNA in PX458 vectorDepositorAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K5Q-GS3
Plasmid#204746PurposeExpression of Cas9 and human H2AX with the K5Q mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K5R-GS3
Plasmid#204747PurposeExpression of Cas9 and human H2AX with the K5R mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134L-GS3
Plasmid#204750PurposeExpression of Cas9 and human H2AX with the K134L mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134M-GS3
Plasmid#204751PurposeExpression of Cas9 and human H2AX with the K134M mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134A-GS3
Plasmid#204752PurposeExpression of Cas9 and human H2AX with the K134A mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-KRAS-G121C/G13A-nick sgRNA
Plasmid#214099PurposeLentiviral vector expressing nicking sgRNA to induce KRAS G12C or G13A mutation using PE3DepositorInsertKRAS G12C or G13A nicking sgRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA233
Plasmid#215941PurposeFragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNADepositorHas ServiceCloning Grade DNAInsertU6_v1; sgCD274_v1 [Sp]; trRNA_v14 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dyn1 R271E D291R
Plasmid#198342PurposeMammalian expression plasmid of GFP-chimera proteins.DepositorAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dyn1 R271E
Plasmid#198343PurposeMammalian expression plasmid of GFP-chimera proteins.DepositorAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dyn1 R271E E720R
Plasmid#198344PurposeMammalian expression plasmid of GFP-chimera proteins.DepositorAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dyn1 K44A
Plasmid#198340PurposeMammalian expression plasmid of GFP-chimera proteins.DepositorAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dyn1 D406A
Plasmid#198341PurposeMammalian expression plasmid of GFP-chimera proteins.DepositorAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-SHTN1LRRR>GGG
Plasmid#209562PurposeMammalian expression of Shtn1 long isoform with RG mutationDepositorInsertShtn1 long isoform with RG mutation (4930506M07Rik Mouse)
ExpressionMammalianMutationRRR to GGGAvailable SinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPAP_scFv_V50A
Plasmid#183734PurposeExpresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signalDepositorInsertAnti-lysozyme scFv
TagsHis6-tagExpressionYeastMutationV50A mutation in MFalpha signalPromoterAOX1 promoterAvailable SinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnTS-E1015A-E1021A
Plasmid#213417PurposeVinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).DepositorInsertVcnTS-E1015A-E1021A (VCL Chicken, Synthetic)
ExpressionMammalianMutationMutated vinculin glutamic acid 1015 and 1021 to a…PromoterCMVAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnCS-E1015A-E1021A
Plasmid#213414PurposeVinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).DepositorInsertVcnCS-E1015A-E1021A (VCL Chicken, Synthetic)
ExpressionMammalianMutationMutated vinculin glutamic acid 1015 and 1021 to a…PromoterCMVAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_Promoter_5
Plasmid#210632PurposeLuciferase experiment for TAL1 promoter 5DepositorAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHA1.1-COMMD3_CDS-V5-hygro
Plasmid#195509Purposelentiviral vector expressing COMMD3 CDS with hygromycin selectionDepositorAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only