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pPLS24

Plasmid

#17285

Depositor

Nancy Craig

Article

Sharpe et al EMBO J. 1998 Oct 1. 17(19):5822-31.

Insert

TnsD (tnsD E. coli transposon Tn7)

Tags

His-tag

Expression

Bacterial

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
pAAV_BiSSTe10_ChR2_mCherry

Plasmid

#213815

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_S9E10_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Gamma

Plasmid

#177963

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Gamma: L13F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Gamma: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) (S )

Expression

Mammalian

Mutation

L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSrTA1662-toolkit

Plasmid

#154372

Purpose

Expresses SrTA1662 in plants

Depositor

Brande Wulff

Article

Gaurav et al Nat Biotechnol. 2022 Mar;40(3):422-431.

Insert

SrTA1662

Expression

Bacterial and Plant

Promoter

Native promoter

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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FUGW

Plasmid

#14883

Purpose

3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresion

Depositor

David Baltimore

Article

Lois et al Science. 2002 Feb 1. 295(5556):868-72.

Insert

flap-Ub promoter-GFP-WRE

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2007

Availability

Academic Institutions and Nonprofits only

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pJTM-T7-mNeongreen-6xHis

Plasmid

#210868

Purpose

Plasmid used for expressing his--tagged mNeongreen for protein purification.

Depositor

Richard Murray

Article

Meyerowitz et al ACS Synth Biol. 2024 Feb 16;13(2):530-53

Insert

mNeongreen-6xHistag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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EF.PGK.GFP

Plasmid

#17618

Depositor

Linzhao Cheng

Article

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pGTy1mhis3-AI or pGTy1his3-AI

Plasmid

#62228

Purpose

A Ty1 element tagged with the retrotransposition indicator gene his3-AI is fused to the GAL1 promoter on a multicopy URA3-based shuttle plasmid.

Depositor

David Garfinkel

Article

Curcio et al Proc Natl Acad Sci U S A. 1991 Feb 1;88(

Insert

Ty1H3 retrotransposon

Expression

Yeast

Promoter

GAL1

Available Since

Feb. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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p CIneo-RL

Plasmid

#115366

Purpose

Expression vector to produce humanized Renilla luciferase

Depositor

Witold Filipowicz, Ramesh Pillai

Article

Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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SlugMyc_pcDNA3

Plasmid

#31698

Depositor

Paul Wade

Article

Kajita et al Mol Cell Biol. 2004 Sep . 24(17):7559-66

Insert

Slug (SNAI2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV/SB11

Plasmid

#26552

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Expression

Bacterial

Mutation

Methionine 243 to Glutamine Threonine 136 to Arg…

Available Since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pSLQ9663 - ACTB GFP-P2A CLIP Donor

Plasmid

#202688

Purpose

CLIP donor template for integrating GFP into ACTB locus. Encodes GFP with ACTB homology arms.

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

CopGFP

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET_RTX

Plasmid

#102787

Purpose

Expresses the Reverse Transcriptase gene

Depositor

Andrew Ellington

Article

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase

Expression

Bacterial

Promoter

T7 promoter + lac operator

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET_RTX_(exo-)

Plasmid

#102786

Purpose

Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

Depositor

Andrew Ellington

Article

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase with 3'-5' deficient exonuclease

Expression

Bacterial

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAM1303

Plasmid

#40243

Depositor

Susan Golden

Article

Kulkarni et al Mol Microbiol. 1997 Jun;24(6):1131-42.

Insert

Sp/Sm Resistance, neutral site I integration platform for Synechococcus

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pRK2-AraE

Plasmid

#110141

Purpose

Constitutive promoter expressing araE on RK2 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

araE

Expression

Bacterial

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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tdTomato-Farnesyl-5

Plasmid

#58092

Purpose

Localization: Membrane, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

Farnesyl (HRAS Human)

Tags

tdTomato

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

Dec. 23, 2014

Availability

Academic Institutions and Nonprofits only

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LV-TRE-Empty-T2A-dsRedExpress2

Plasmid

#60623

Purpose

Expresses dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

Empty

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9

Plasmid

#137079

Purpose

Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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p2B-56

Plasmid

#177925

Purpose

Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter

Depositor

Vadim Gladyshev

Article

Barre et al (unpublished)

Insert

Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter

Expression

Bacterial and Yeast

Mutation

V9I; F67S; G72P, F86Y; R171K

Promoter

MYO2

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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