We narrowed to 5,112 results for: codon optimized

1. TRE3GV-SpdCas9-Dnmt3A/3L-V1

   Plasmid

   #222503

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A

   Promoter

   TRE3GV and hPGK

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRE3GV-SpdCas9-Dnmt3A/3L-V2

   Plasmid

   #222505

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A

   Promoter

   TRE3GV and hPGK

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TRE3GV-SpdCas9-Dnmt3A/3L-V3

   Plasmid

   #222506

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E and E814…

   Promoter

   TRE3GV and hPGK

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TRE3GV-SpdCas9-Dnmt3A/3L-catΔ

   Plasmid

   #222508

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; C706A and R832…

   Promoter

   TRE3GV and hPGK

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTR-CASK

   Plasmid

   #221427

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   CASK (CASK Human)
   

   Expression

   Mammalian

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCG_SARS-CoV1 C-V5-tag ORF7a

   Plasmid

   #179971

   Purpose

   Mammalian expression vector for SARS-CoV-1 ORF7a, V5-tagged. Sequence codon-optimized.

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   SARS-CoV-1 ORF7a (ORF7a Synthetic)
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-myc-S (P.1 lineage / Brazilian variant)

   Plasmid

   #169847

   Purpose

   Mammalian expression plasmid for S of SARS-CoV-2 (P.1 lineage / Brazilian variant) with N-terminal c-myc tag

   Depositor

   Erik Procko

   Article

   Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

   Insert

   Protein S
   

   Tags

   HA leader and c-myc tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4-myc-S (B.1.1.7 lineage / UK variant)

   Plasmid

   #169848

   Purpose

   Mammalian expression plasmid for S of SARS-CoV-2 (B.1.1.7 lineage / UK variant) with N-terminal c-myc tag

   Depositor

   Erik Procko

   Article

   Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

   Insert

   Protein S
   

   Tags

   HA leader and c-myc tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ287-RVR

   Plasmid

   #138124

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a with N512R, K518V and N522R mutations, without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   BsCas12a RVR variant
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   N512R, K518V and N522R

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ285-RVR

    Plasmid

    #138121

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a with N512R, K518V and N522R mutations, without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    Lb5Cas12a RVR variant
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    N512R, K518V and N522R

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ239-STU

    Plasmid

    #138112

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized FnCas12a without promoter and terminator

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    FnCas12a
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVZ_PcoaT_SQE1_THAS1

    Plasmid

    #103859

    Purpose

    Heterologous, cobalt-inducible expression of SQE1 and THAS1 from A. thaliana in Synechocystis sp. PCC6803

    Depositor

    Ilka Axmann

    Article

    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d

    Insert

    thalianol synthase 1 (THAS1 Mustard Weed)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    inserted genes are codon optimized. 2nd amino aci…

    Promoter

    PcoaT (from Synechocystis sp. PCC 6803)

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSP1-bio

    Plasmid

    #47709

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP1 (MSP1 Plasmodium falciparum, Synthetic)
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV6-AN-DDK_WT POLI

    Plasmid

    #131228

    Purpose

    Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Coding sequence has been codon optimised for expr…

    Promoter

    CMV enhancer + CMV promoter

    Available Since

    Dec. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX

    Plasmid

    #154910

    Purpose

    1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.

    Depositor

    Jonathan Clarke, Clare Buckley

    Article

    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

    Insert

    Phytochrome B (PHYB Synthetic, Mustard Weed)
    

    Use

    Xenopus/avian/zebrafish

    Tags

    CAAX membrane moiety and mCherry

    Expression

    Mammalian

    Mutation

    1-621 amino acids of Arabidopsis PHYB

    Promoter

    CMV

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV6-AN-DDK_UBM1*2* POLI

    Plasmid

    #131232

    Purpose

    Mammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domains

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains inactivating mutations in both ubiquitin…

    Promoter

    CMV enhancer + CMV promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PF3D7_1136200-bio

    Plasmid

    #47730

    Purpose

    Expresses enzymatically monobiotinylated full-length PF3D7_1136200 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised PF3D7_1136200
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin replaced…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EBL1-bio

    Plasmid

    #47792

    Purpose

    Expresses enzymatically monobiotinylated full-length EBL1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised EBL1
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TLP-bio

    Plasmid

    #47787

    Purpose

    Expresses enzymatically monobiotinylated full-length TLP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised TLP
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PF3D7_1431400-bio

    Plasmid

    #47791

    Purpose

    Expresses enzymatically monobiotinylated full-length PF3D7_1431400 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised PF3D7_1431400
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge