We narrowed to 5,692 results for: org

1. pLib6.4B

   Plasmid

   #176665

   Purpose

   Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-HsSENP7

   Plasmid

   #71470

   Purpose

   Encodes human full length SENP7. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 7 (SENP7 Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pSMP-SMYD2_1

   Plasmid

   #36349

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   SMYD2 (SMYD2 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pLib6.6B

   Plasmid

   #176666

   Purpose

   Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-flag-UTR

   Plasmid

   #183234

   Purpose

   Mammalian expression empty backbone

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Type

   Empty backbone

   Tags

   FLAG tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. ER-G-Ca-FLITS-KDEL

   Plasmid

   #191464

   Purpose

   Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   G-Ca-FLITS
   

   Tags

   ER and KDEL

   Expression

   Mammalian

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHJ18

   Plasmid

   #48357

   Purpose

   Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectable

   Depositor

   George Cross

   Article

   Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

   Insert

   Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR
   

   Use

   Cre/Lox; Knockout in t. brucei

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRAN334

   Plasmid

   #120814

   Purpose

   E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)

   Depositor

   Craig Ellermeier, David S. Weiss

   Article

   Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

   Insert

   cyan fluorescent protein, codon-optimized for low GC organisms
   

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHJ41

   Plasmid

   #48360

   Purpose

   Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectable

   Depositor

   George Cross

   Article

   Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

   Insert

   Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR
   

   Use

   Cre/Lox; Knockout in t. brucei

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSR35

    Plasmid

    #69256

    Purpose

    phsp16.41::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    hsp16.41

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSR28

    Plasmid

    #69253

    Purpose

    phlh-8::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    hlh-8

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSMP-SMYD2_2

    Plasmid

    #36350

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    SMYD2 (SMYD2 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

13. gRNA_tdtomato_reporter_1_ST1_2x_binding

    Plasmid

    #79368

    Purpose

    Assays activity of transcriptional activators fused to ST1 Cas9

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    tdtomato
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. dSp-VP64-p65(150-261)-RTA(125-190)

    Plasmid

    #99674

    Purpose

    Expresses dSp Cas9 fused to combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    VP64-P65(151-261)-RTA(125-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSMP-SMYD2_3

    Plasmid

    #36351

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    SMYD2 (SMYD2 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pRSV-p65T305A,S276A

    Plasmid

    #106456

    Purpose

    NF-kB p65, kinase sites 276,305 mutated

    Depositor

    Georg Weber

    Article

    He et al Eur J Biochem. 2003 May . 270(10):2174-8

    Insert

    NF-kB p65, kinase sites 276,305 mutated (RELA Human)
    

    Expression

    Mammalian

    Mutation

    kinase sites inactivated

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHJ32

    Plasmid

    #48361

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3_3_TOPO_TA-ms2-VP64

    Plasmid

    #79371

    Purpose

    Recruits VP64 to a compatible gRNA for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    MS2-VP64
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-SMVP-Cas9N-U6-gRNA M4

    Plasmid

    #80937

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA M4 for Mstn cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSR21

    Plasmid

    #69156

    Purpose

    read-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    TagBFP
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    rps-27

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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