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(203-bp-TET-DIAL-CMV53)-mGL-bGH_pDA046

Plasmid

#246365

Purpose

203-bp DIAL Reporter Plasmid with CMV53 expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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(203-bp-TET-DIAL-MLP)-mGL-bGH_pDA047

Plasmid

#246366

Purpose

203-bp DIAL Reporter Plasmid with MLP expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_((203-bp-DIAL-YB_TATA)-mGL-BGH)rev_DIV_pKG2948

Plasmid

#246343

Purpose

203-bp DIAL Reporter Lentivirus (with divergent iRFP670) expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Lentiviral and Synthetic Biology

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_((380-bp-DIAL)-mCh-HRasG12V-BGH)rev_DIV_pKG3848

Plasmid

#246344

Purpose

380-bp DIAL (YB_TATA) Reporter Lentivirus (with divergent SNAP) expressing mCherry-HRasG12V in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Lentiviral and Synthetic Biology

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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(nested-DIAL-YB_TATA)-mGL-BGH_pKG2876

Plasmid

#246332

Purpose

nested DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre and VCre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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(203-bp-DIAL-minCMV)-mGL-BGH_pKG3654

Plasmid

#246333

Purpose

203-bp DIAL Reporter Plasmid with minCMV expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-SV40)-mGL-BGH_pKG3687

Plasmid

#246334

Purpose

203-bp DIAL Reporter Plasmid with SV40 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-minCMV2)-mGL-BGH_pKG3680

Plasmid

#246335

Purpose

203-bp DIAL Reporter Plasmid with minCMV2 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-JB42CAT5)-mGL-BGH_pKG3688

Plasmid

#246336

Purpose

203-bp DIAL Reporter Plasmid with JB42CAT5 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-MLP)-mGL-BGH_pKG3681

Plasmid

#246337

Purpose

203-bp DIAL Reporter Plasmid with MLP expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-CMV53)-mGL-BGH_pKG3682

Plasmid

#246338

Purpose

203-bp DIAL Reporter Plasmid with CMV53 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-YB_TATA)-mGL-BGH_pKG1525

Plasmid

#246326

Purpose

203-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 PCSK5(M452I)

Plasmid

#232445

Purpose

Gateway pDONR vector

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(M452I) (PCSK5 Human)

Use

Gateway entry vector

Mutation

M452I

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLX304 PCSK5(M452I)-V5 blast

Plasmid

#232446

Purpose

Lentiviral expression vector for PCSK5(M452I)

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(M452I) (PCSK5 Human)

Use

Lentiviral

Tags

V5

Mutation

M452I

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEN_TT PCSK5-V5

Plasmid

#232448

Purpose

Gateway entry vector for an inducible PCSK5

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5 (PCSK5 Human)

Use

Gateway entry vector

Tags

V5

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEN_TT PCSK5(sgRR)-V5

Plasmid

#232449

Purpose

Gateway entry vector for an inducible sg09 resistant PCSK5

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5 (PCSK5 Human)

Use

Gateway entry vector

Tags

V5

Mutation

a75g_t84c_g87c

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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EDCPV PCSK5_sg09

Plasmid

#232455

Purpose

Lentiviral, CRISPR sgRNA for PCSK5

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5 sgRNA (PCSK5 Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEf1a-Dn29-P2A-GFP

Plasmid

#247151

Purpose

Mammalian expression of a human codon optimized wildtype Dn29 recombinase

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1014

Plasmid

#239936

Purpose

Hsp18.2 (heat inducible) promoter driving the expression of sgRNA-B

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

Hsp18.2::sgRNA-B

Use

Synthetic Biology

Expression

Plant

Mutation

N/A

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-HyPerFLEX-NLSx3-WPRE

Plasmid

#217563

Purpose

Mammalian expression of HyPerFLEX targeted to the nucleus

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-NLS

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CaMKII-HyPerFLEX-UnaG-WPRE

Plasmid

#217564

Purpose

Expression of HyPerFLEX fused with UnaG in mammalian neurons

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-UnaG

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-CMV-HyPerFLEX-UnaG-WPRE

Plasmid

#217565

Purpose

Mammalian expression of HyPerFLEX fused with UnaG

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-UnaG

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-CAG-HyPerFLEX-NES-WPRE

Plasmid

#217562

Purpose

Mammalian expression of HyPerFLEX targeted to the cytoplasm

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-NES

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-HyPerFLEX-Sec61b

Plasmid

#238940

Purpose

Mammalian expression of HyPerFLEX targeted to the cytosolic side of the endoplasmic reticulum membranes

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-Sec61b

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pC1-HyPerFLEX-ERlum

Plasmid

#238941

Purpose

Mammalian expression of HyPerFLEX targeted to the luminal side of the endoplasmic reticulum membranes

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-ERlum (Calreticulin signal sequence-HyPerFLEX-KDEL)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TVMV-HRV-3C Proteases-mCherry

Plasmid

#243796

Purpose

Encodes for mammalian expression of TVMV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

TVMV and HRV-3C proteases

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
mGreenLantern-[(A OR S) AND T] OR (C AND V)-SpyTag

Plasmid

#243789

Purpose

Expresses multifunctional construct enabling the [AND/(OR)]OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9), sortase eSrtA(4S9), TEV, HRV-3C, TEV and TVMV proteases. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag and SsrA

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TVMV-TEV Proteases-mCherry

Plasmid

#243797

Purpose

Encodes for mammalian expression of TVMV and TEV protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

TVMV and TEV proteases

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP984

Plasmid

#239906

Purpose

TCTP-SynPro-04 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-04 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP985

Plasmid

#239907

Purpose

TCTP-SynPro-05 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-05 engineered promoter

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge