We narrowed to 7,724 results for: Pol
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Plasmid#210925PurposeInsect expression of human SEC31A fragment M1 to T338. N-terminal 6X His tag with TEV cleavage.DepositorInsertSEC31A:M1-T338 (SEC31A Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_RFWD3:425-774
Plasmid#210884PurposeInsect expression of human RFWD3 fragment S425 to E774. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertRFWD3:S425-E774 (RFWD3 Human)
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving
Plasmid#204811PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
ExpressionBacterialAvailable sinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-Lyn-EGFP
Plasmid#196875PurposeNeuron-specific expression of LynGFP reporterDepositorInsertLynEGFP
UseCre/LoxExpressionMammalianPromoterQuimeric CAGAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-Nedd1-mOrange2-rCM1
Plasmid#196861PurposeExpression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.DepositorInsertNedd1-mOrange2-rCM1 (Nedd1 Rat)
ExpressionMammalianPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
AM77_pU6.Sa-gRNA.CLYBL
Plasmid#199237PurposeExpression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locusDepositorInsertS. aureus gRNA spacer
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterRNA polymerase III promoter for human U6 snRNAAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-KIF13aMotor-mScarlet-FKBP
Plasmid#191337PurposeExpresses the fluorescently labeled plus-end directed KIF13a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKIF13aMotor-mScarlet-FKBP (KIF13A Human)
ExpressionMammalianMutationmotor domain aa 1-639Available sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
C211
Plasmid#174360PurposeFor expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfectionDepositorInsertPABPC1 (PABPC1 Human)
TagsFLAGExpressionMammalianMutationI110L, D111E, D117E, A121G, G139A, Y140F, T147S, …Available sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Nedd4.2F823A
Plasmid#165104Purposebacterial expression of a GST fusion of Human Nedd4.2d F823ADepositorInsertNedd4.2d F823A (NEDD4L Human)
TagsGST from plasmid.ExpressionBacterialMutationF823A; mutation for destabilization of trimer fo…Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa.NgCAM-Halo
Plasmid#162719PurposeMammalian expression of chicken NgCamDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNgCam (L1CAM Chicken)
TagsHaloExpressionMammalianMutationE137K, K138E, and K642EPromoterChicken Beta actinAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4134323
Plasmid#152878PurposeMammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;TapTagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1(+)_UG7
Plasmid#135990PurposeExpresses UG7 in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUtrCH
Tagshelical linker-mEGFPExpressionMammalianPromoterCMV promoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PRK2A I12_pECIA14
Plasmid#114787PurposePrey vector PRK2A I12_pECIA14 should be used with bait vector PRK2A I12_pECIA2.DepositorAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC210
Plasmid#118896Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3CDepositorInsertNcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
TagsMBP, cleavable with 3C protease and SNAP tag, cle…ExpressionInsectPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 T292A L293A P294A
Plasmid#112104PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDynamin1 (DNM1 Human)
TagsEGFPExpressionMammalianMutationT292A L293A P294APromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 L330R Q334R L702R
Plasmid#112105PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDynamin1 (DNM1 Human)
TagsEGFPExpressionMammalianMutationL330R Q334R L702RPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406R M407R T488W
Plasmid#112111PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDynamin1 (DNM1 Human)
TagsEGFPExpressionMammalianMutationD406R M407R T488WPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E3HCA8
Plasmid#103100PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE3HCA8(Cas9 coding gene from Ilyobacter polytropus (strain DSM 2926 / CuHBu1))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC
Plasmid#99649PurposeBacterial expression of wildtype Rag GTPase dimerDepositorTags10x His tag, Poly arginine linker, and SUMOExpressionBacterialPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(Q120L)
Plasmid#99701PurposeBacterial expression of RagA-RagC(Q120L)DepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationQ120LPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only