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EKAR2G_design2_mTFP_175_Venus_229

Plasmid

#40013

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only
pGSTag BimEL (T112A)

Plasmid

#23101

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TA) (Bcl2l11 Mouse)

Tags

Gst

Expression

Bacterial

Mutation

Changed Threonine 112 to Alanine

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pGSTag BimEL (T112D)

Plasmid

#23102

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TD) (Bcl2l11 Mouse)

Tags

Gst

Expression

Bacterial

Mutation

Changed Threonine 112 to Aspartate

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
Wnt8A STOP

Plasmid

#35879

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8A (Wnt8a Human)

Use

Entry vector

Mutation

Silent Mutation A (600) --> G

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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EKAR2G_design2_mTFP_105_Venus_157

Plasmid

#40000

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
EKAR2G_design1_mTFP_175_Venus_wt

Plasmid

#39828

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
EKAR2G_design1_mTFP_227_Venus_wt

Plasmid

#39833

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
EKAR2G_design1_mTFP_159_Venus_wt

Plasmid

#39823

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pGL3-p107 2*

Plasmid

#32388

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

2nd E2F binding site mutated: GTC-->AAA

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
puDNA5_SSF_DOR_GLUT4_LEYL_APEX2

Plasmid

#250768

Purpose

Mammalian cell expression of the delta opioid receptor with the last 17 amino acids of GLUT4

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

DOR-GLUT4(LEYL)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Mutation

delta opioid receptor with last 17 amino acids of…

Promoter

UBC

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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puDNA5_SSF_DOR_MRS_WT_APEX2

Plasmid

#250762

Purpose

Mammalian cell expression of the delta opioid receptor with the last 17 amino acids of the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

DOR-MRS(WT)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Mutation

delta opioid receptor with last 17 amino acids of…

Promoter

UBC

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pBABE-SV40-puro-T2A-TagBFP

Plasmid

#254075

Purpose

Retroviral transfer empty vector, puromycin selection marker.

Depositor

Jun Huh

Article

Lu et al Cell. 2026 Feb 5;189(3):939-955.e26. doi

Type

Empty backbone

Use

Retroviral

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV_aCatTS_indicator

Plasmid

#252386

Purpose

Non-FRET aCatenin tension indicator. cpEGFP with elastic linker is inserted at the hinge with C-term mCherry.

Depositor

Maretoshi Hirai

Article

Fujiwara et al Commun Biol. 2026 Feb 19. doi: 10.1038/s

Insert

aCatenin tension indicator

Tags

mCherry

Expression

Mammalian

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_aActTS_indicator

Plasmid

#252385

Purpose

Non-FRET aActinin tension indicator. cpEGFP with elastic linker is inserted at the hinge with C-term mCherry.

Depositor

Maretoshi Hirai

Article

Fujiwara et al Commun Biol. 2026 Feb 19. doi: 10.1038/s

Insert

aActinin tension indicator

Tags

mCherry

Expression

Mammalian

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pBR25 CFAP36 [L438E, K442E]-3C-mStayGold-2Strep crBSDr (C. reinhardtii)

Plasmid

#238227

Purpose

CRISPR donor plasmid for C-terminal tagging and knock-in of CFAP36 [L438E, K442E], and insertion of Blasticidin resistance (C. reinhardtii)

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [L438E, K442E]

Use

CRISPR; Donor

Tags

-3C-mStayGold-2Strep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pBR25 CFAP36 [A493E, K495A]-3C-mStayGold-2Strep crBSDr (C. reinhardtii)

Plasmid

#238228

Purpose

CRISPR donor plasmid for C-terminal tagging and knock-in of CFAP36 [A493E, K495A], and insertion of Blasticidin resistance (C. reinhardtii)

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [A493E, K495A]

Use

CRISPR; Donor

Tags

-3C-mStayGold-2Strep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pBR25 IFT70 [A331R, A335R] crSpecR (C. reinhardtii)

Plasmid

#238230

Purpose

CRISPR donor plasmid for C-terminal knock-in of IFT70 [A331R, A335R] and insertion of Spectinomycin resistance (C. reinhardtii)

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

IFT70 [A331R, A335R]

Use

CRISPR; Donor

Tags

None

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA0650

Plasmid

#240487

Purpose

CRISPRi-mediated Knockdown of RPA0650

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA3717

Plasmid

#240491

Purpose

CRISPRi-mediated Knockdown of RPA3717

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA3701

Plasmid

#240490

Purpose

CRISPRi-mediated Knockdown of RPA3701

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge